Chen Y, Wang Y, Jiang S, Zhang J, Lü Z, Deng S
College of Marine Science and Technology, Zhejiang Ocean University, Changzhi Island, Zhoushan, Zhejiang, 316022, China.
College of Food and Pharmacy, Zhejiang Ocean University, Changzhi Island, Zhoushan, Zhejiang, 316022, China.
Anim Genet. 2018 Oct;49(5):472-474. doi: 10.1111/age.12676. Epub 2018 Jul 5.
The large yellow croaker, Larimichthys crocea, is a commercially important drum fish (Family: Sciaenidae) native to the East and South China Sea. Habitat deterioration and overfishing have led to significant population decline and the collapse of its fishery over the past decades. Today, the market supply of L. crocea depends solely on stocks produced in hatcheries and farms. Common issues that occur in the culture of L. crocea include germplasm degradation, precocious puberty, elevated disease susceptibility and growth retardation. In this study, we employed SLAF-seq (specific-locus amplified fragment sequencing) technology to identify single nucleotide polymorphism (SNP) loci across the L. crocea genome. Sixty samples were selected for SLAF analysis out of 1000 progeny in the same cohort of a cultured stock. Our analysis obtained a total of 151 253 SLAFs, of which 65.88% (99 652) were identified to be polymorphic, scoring a total of 710 567 putative SNPs. Further filtration resulted in a final panel of 1782 SNP loci. The data derived from this work could be beneficial for understanding the genetics of complex phenotypic traits as well as for developing marker-selection-assisted breeding programs in L. crocea.
大黄鱼（Larimichthys crocea）是一种具有重要商业价值的石首鱼科鱼类，原产于中国东海和南海。在过去几十年中，栖息地退化和过度捕捞导致大黄鱼种群数量大幅下降，其渔业资源也随之崩溃。如今，大黄鱼的市场供应完全依赖于孵化场和养殖场的养殖种群。大黄鱼养殖过程中常见的问题包括种质退化、早熟、疾病易感性增加和生长迟缓。在本研究中，我们采用了特异性位点扩增片段测序（SLAF-seq）技术来鉴定大黄鱼全基因组中的单核苷酸多态性（SNP）位点。从同一养殖群体的1000个后代中选取了60个样本进行SLAF分析。我们的分析共获得了151253个SLAF，其中65.88%（99652个）被鉴定为多态性位点，总计获得710567个潜在SNP。进一步筛选后得到了最终的1782个SNP位点面板。这项工作所获得的数据有助于理解复杂表型性状的遗传学，以及开展大黄鱼的标记辅助选择育种计划。
