Luquette M H, Kimball P M, Pretlow T P, Herrera G A, Pretlow T G
Lab Invest. 1985 Nov;53(5):546-55.
F344 male rats were given 90 ppm diethylnitrosamine in their drinking water ad libitum in two cycles. Livers containing neoplastic nodules, hepatomas, and no sarcomas in the sections sampled were digested in parallel with 0.05% collagenase, 0.1% Pronase, or 0.25% trypsin. Cells were transplanted into 9- to 19-day-old F344 rats. Despite the absence of sarcomas in the sections examined microscopically from each such liver before digestion and the presence of multiple hepatomas in all sections examined, vascular sarcomas, probably angiosarcomas, were observed in a large proportion of animals injected with the suspensions of cells; hepatomas were not observed in these animals. Morphology by light microscopy, immunohistochemical demonstration of factor VIII, histochemical demonstration of alkaline phosphatase, and the presence of Weibel-Palade bodies strongly suggest that these tumors are angiosarcomas. Similar tumors developed from cells obtained in parallel with the aid of Pronase, collagenase, or trypsin. Cell suspensions obtained with Pronase yielded tumors with the shortest latent period between the injection of cells and the death of one-half of the transplant recipients. The procedure that we used provides a consistent method for the production of transplantable sarcomas. The absence of sarcomas in the single sections taken from donor livers and multiple sections of similar livers not used for transplantation suggests that transplantability of these sarcoma cells is acquired very early in this neoplasm.
将90 ppm的二乙基亚硝胺随意添加到F344雄性大鼠的饮用水中，分两个周期进行。对切片中含有肿瘤结节、肝癌且无肉瘤的肝脏，分别用0.05%的胶原酶、0.1%的链霉蛋白酶或0.25%的胰蛋白酶进行平行消化。将细胞移植到9至19日龄的F344大鼠体内。尽管在消化前从每个这样的肝脏显微镜检查的切片中未发现肉瘤，且在所有检查的切片中均存在多个肝癌，但在注射细胞悬液的大部分动物中观察到了血管肉瘤，可能是血管内皮肉瘤；在这些动物中未观察到肝癌。光镜形态学、因子VIII的免疫组化证明、碱性磷酸酶的组织化学证明以及魏贝尔-帕拉德小体的存在强烈提示这些肿瘤是血管内皮肉瘤。用链霉蛋白酶、胶原酶或胰蛋白酶平行获得的细胞也产生了类似的肿瘤。用链霉蛋白酶获得的细胞悬液产生的肿瘤，在注射细胞与一半移植受体死亡之间的潜伏期最短。我们使用的方法为产生可移植肉瘤提供了一种一致的方法。从供体肝脏获取的单个切片以及未用于移植的类似肝脏的多个切片中未发现肉瘤，这表明这些肉瘤细胞的可移植性在该肿瘤形成的早期就已获得。
