Li Liang, Chen Hui-Xia, Yang Yue
Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, 050024 Shijiazhuang, Hebei Province, People's Republic of China.
Acta Parasitol. 2018 Sep 25;63(3):479-485. doi: 10.1515/ap-2018-0057.
In the present study, Neorhadinorhynchus nudus (Harada, 1938) is reported from the frigate tuna Auxis thazard (Lacepéde) (Perciformes: Scombridae), in the South China Sea for the first time. The detailed morphology of N. nudus was studied using light and scanning electron microscopy based on the newly collected material. The results showed some morphometric variability between our specimens and previous studies, including the number of hooks per longitudinal row and the size of copulatory bursa and eggs. Our SEM observations also revealed all proboscis hooks emerged from elevated round rims on proboscis surface. In addition, N. nudus was firstly characterised using molecular methods by sequencing and analysing the ribosomal ITS and mitochondrial cox1 regions. There is no nucleotide divergence found in the ITS sequences, but a low level of nucleotide variability detected in the cox1 regions (the level of intraspecific nucleotide variability being 0.75% to 2.54%). The DNA sequence data obtained herein will indeed be a useful reference for rapid and accurate species identification of Neorhadinorhynchus.
在本研究中，首次在中国南海的扁舵鲣（Auxis thazard）（鲈形目：鲭科）中报道了裸新棘吻虫（Neorhadinorhynchus nudus）（原田，1938年）。基于新采集的材料，利用光学显微镜和扫描电子显微镜研究了裸新棘吻虫的详细形态。结果表明，我们的标本与先前研究之间存在一些形态测量上的差异，包括每纵行的钩数以及交配囊和卵的大小。我们的扫描电子显微镜观察还显示，所有吻钩都从吻表面凸起的圆形边缘长出。此外，首次通过对核糖体ITS和线粒体cox1区域进行测序和分析，利用分子方法对裸新棘吻虫进行了特征描述。在ITS序列中未发现核苷酸差异，但在cox1区域检测到低水平的核苷酸变异（种内核苷酸变异水平为0.75%至2.54%）。本文获得的DNA序列数据确实将成为新棘吻虫快速准确物种鉴定的有用参考。
