Gorgels-Kallen Janine L, Meij Johanna T A
Zoölogisch Laboratorium, Faculteit der Wiskunde en Natuurwetenschappen, Katholieke Universiteit, Toernooiveld, 6525 ED Nijmegen, The Netherlands.
J Morphol. 1985 Feb;183(2):155-163. doi: 10.1002/jmor.1051830204.
Immunocytochemical investigation of the eyestalks of the larval and postlarval stages of the crayfish Astacus letodactylus, using an anti-Astacus-CHH serum, shows that immunopositive staining starts in the immediate posthatching larval stage, in neurosecretory cells of the medulla terminalis ganglionic X-organ (MTGX), in an MTGX-sinus gland tract, and in part of the axon terminals of the sinus gland. The number of these immunopositive cells increases during postlarval development. Neurosecretory granules belonging to distinct axon terminals of the neurohemal region show immunostaining. The mean diameter of the stained granules increases during postlarval development. The hemolymph glucose concentration in adult crayfish increases after injection of larval eyestalks.
使用抗螯虾促性腺激素释放激素（Astacus-CHH）血清对螯虾Astacus letodactylus幼体和成体后期眼柄进行免疫细胞化学研究，结果表明，免疫阳性染色始于孵化后即刻的幼体阶段，位于终髓神经节X器官（MTGX）的神经分泌细胞、MTGX-窦腺束以及窦腺的部分轴突末端。这些免疫阳性细胞的数量在幼体后期发育过程中增加。属于神经血器官区域不同轴突末端的神经分泌颗粒呈现免疫染色。染色颗粒的平均直径在幼体后期发育过程中增大。注射幼体眼柄后，成年螯虾的血淋巴葡萄糖浓度升高。
