宫颈癌保留神经根治性子宫切除术中盆腔自主神经的蛋白质组学分析
Proteomic Analysis of Pelvic Autonomic Nerve in Nerve-sparing Radical Hysterectomy for Cervical Carcinoma.
作者信息
Lee Yoon Hee, Kim Min Kyung, Moon Hee Young, Chong Gun Oh, Lee Hyun Jung, Lee Yoon Soon, Park Ji Young, Lee Chan Hyeong, Baek Moon Chang, Hong Dae Gy
机构信息
Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, Republic of Korea.
Department of Pathology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, Republic of Korea.
出版信息
Cancer Genomics Proteomics. 2018 Jul-Aug;15(4):337-342. doi: 10.21873/cgp.20092.
BACKGROUND/AIM: This study was designed to identify candidate proteins which can be used for visualization of pelvic autonomic nerve during nerve-sparing surgery.
MATERIALS AND METHODS
Both soft tissue and vesical branch of the inferior hypogastric nerve from five women were collected during surgery. These 10 tissue specimens were analysed using liquid chromatography-mass spectrometry (LC-MS) and Human Protein Atlas (HPA) for protein expression. The existence of nerve fibres was confirmed using haematoxylin and eosin (H&E) and anti-S-100 staining.
RESULTS
A total of 413 proteins were detected. There were three proteins (isoform 1 of fibronectin, protein S100-A8 and A9) which implied a relation with pelvic autonomic nerve. In nerve tissue from one case, the existence of nerve fibre was not confirmed.
CONCLUSION
Further large studies are expected to present more nerve-specific candidate proteins which can be used for the easy and safe identification of autonomic nerves.
背景/目的：本研究旨在鉴定可用于保留神经手术中盆腔自主神经可视化的候选蛋白。
材料与方法
手术过程中收集了5名女性的下腹下神经的软组织和膀胱分支。使用液相色谱-质谱联用仪（LC-MS）和人类蛋白质图谱（HPA）对这10个组织标本进行蛋白质表达分析。使用苏木精和伊红（H&E）染色及抗S-100染色确认神经纤维的存在。
结果
共检测到413种蛋白质。有三种蛋白质（纤连蛋白异构体1、蛋白质S100-A8和A9）暗示与盆腔自主神经有关。在1例的神经组织中，未确认神经纤维的存在。
结论
预计进一步的大型研究将呈现更多可用于轻松、安全识别自主神经的神经特异性候选蛋白。
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