阿片类药物和纳洛酮对蛙脊髓膜组分中钠钾-三磷酸腺苷酶活性的影响。

The effect of opioids and of naloxone on Na+,K+-adenosine triphosphatase activity in frog spinal cord membrane fractions.

作者信息

Hájek I, Teisinger J, Syková E

出版信息

Neurosci Lett. 1985 Sep 6;59(3):291-5. doi: 10.1016/0304-3940(85)90147-8.

Abstract

The effects of opioids and of naloxone on ouabain-sensitive Na+,K+-adenosine triphosphatase (ATPase) activity were studied in vitro on membrane fractions from frog spinal cords. The addition of morphine and of the stable enkephalin analogue, D-Ala2,D-Leu5-enkephalin, in concentrations from 10(-7) to 10(-4) M significantly increased Na+,K+-ATPase activity. No effect was found with methionine enkephalin (Met-Enk). However, the addition of two peptidase inhibitors, captopril and phosphoramidon (10(-5) M each), significantly increased Na+,K+-ATPase activity. A further increase in enzyme activity was found when Met-Enk (10(-4) or 10(-7) M) was added simultaneously with peptidase inhibitors. On the other hand, the addition of the opiate antagonist, naloxone, at low concentration (10(-7) M) decreased the activity of Na+,K+-ATPase. These results are discussed with respect to the effect of synthetic and endogenous opioids on the activity of Na+,K+-ATPase.

摘要

在体外对青蛙脊髓的膜组分研究了阿片类药物和纳洛酮对哇巴因敏感的Na⁺,K⁺-腺苷三磷酸酶（ATP酶）活性的影响。添加浓度为10⁻⁷至10⁻⁴M的吗啡和稳定的脑啡肽类似物D-Ala²,D-Leu⁵-脑啡肽可显著增加Na⁺,K⁺-ATP酶活性。甲硫氨酸脑啡肽（Met-Enk）未发现有此作用。然而，添加两种肽酶抑制剂卡托普利和磷酰胺素（各10⁻⁵M）可显著增加Na⁺,K⁺-ATP酶活性。当Met-Enk（10⁻⁴或10⁻⁷M）与肽酶抑制剂同时添加时，发现酶活性进一步增加。另一方面，添加低浓度（10⁻⁷M）的阿片拮抗剂纳洛酮可降低Na⁺,K⁺-ATP酶的活性。就合成和内源性阿片类药物对Na⁺,K⁺-ATP酶活性的影响对这些结果进行了讨论。