Abbas Hisham A, El-Ganiny Amira M, Kamel Hend A
Microbiology and Immunology Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.
Microbiology Department, Faculty of Pharmacy and Pharmaceutical Industries, Sinai University, Kantara, Egypt.
Afr Health Sci. 2018 Mar;18(1):11-21. doi: 10.4314/ahs.v18i1.3.
is a major nosocomial uropathogen. It can tolerate a wide variety of physical conditions and many antibiotics by different resistance mechanisms.
This study aimed to investigate the mechanisms of antibiotics resistance in uropathogenic clinical isolates.
Two hundred sixty six urine samples were collected from Zagazig University Hospitals, Zagazig, Egypt. isolates were identified using standard microbiological tests. The sensitivity to different antibiotics was determined by disc diffusion method. Anti-microbial resistance mechanisms were investigated using phenotypic methods and confirmed by PCR.
Fifty isolates were recovered. All isolates were MDR and were resistant to amoxicillin/clavulinic, sulphamethaxzole/trimethoprim, doxycycline and ceftazidime. Phenotypic detection of resistance mechanisms revealed that all strains have efflux mechanism, outer membrane porins, and AmpC β-lactamase; none of the strains showed ESBL activity and two of the imipenem resistant strains showed MβL activity. PCR analysis showed that all strains have MexAB-R, OprD and AmpC genes, 42 strains had PSE gene, while VEB and VIM genes were not detected.
The resistance rates in were higher than global values; this resistance was attributed to several mechanisms. This high resistance is alarming and necessitates applying strict antibiotic prescription policies.
是一种主要的医院内尿路致病菌。它能够通过不同的耐药机制耐受多种物理条件和许多抗生素。
本研究旨在调查尿路致病性临床分离株的抗生素耐药机制。
从埃及扎加齐格大学医院收集了266份尿液样本。使用标准微生物学检测方法鉴定分离株。通过纸片扩散法测定对不同抗生素的敏感性。使用表型方法研究抗菌耐药机制，并通过聚合酶链反应（PCR）进行确认。
共分离出50株菌株。所有分离株均为多重耐药，对阿莫西林/克拉维酸、磺胺甲恶唑/甲氧苄啶、强力霉素和头孢他啶耐药。耐药机制的表型检测显示，所有菌株均有外排机制、外膜孔蛋白和AmpCβ-内酰胺酶；没有菌株显示超广谱β-内酰胺酶（ESBL）活性，2株对亚胺培南耐药的菌株显示金属β-内酰胺酶（MβL）活性。PCR分析表明，所有菌株均有MexAB-R、OprD和AmpC基因，42株有PSE基因，未检测到VEB和VIM基因。
的耐药率高于全球水平；这种耐药性归因于多种机制。这种高耐药性令人担忧，需要实施严格的抗生素处方政策。
