Powell C T, Ney C, Aran P, Agarwal K
Nucleic Acids Res. 1985 Oct 25;13(20):7299-305. doi: 10.1093/nar/13.20.7299.
By employing S1 nuclease mapping of total RNA isolated from porcine cerebral cortex, cerebellum, hypothalamus, pituitary, kidney, liver, pancreas, intestine, and antral mucosa, we have investigated gastrin gene expression in these tissues. Our results show that a gastrin gene is expressed only in the antral mucosal and pituitary tissues. Based on the amount of gastrin specific probe protected from S1 nuclease digestion in the presence of a given weight of total RNA, the amount of gastrin mRNA present in pituitary is approximately 330 times lower than in antral mucosa. These findings help establish the tissue distribution of gastrin gene expression.
通过对从猪大脑皮层、小脑、下丘脑、垂体、肾脏、肝脏、胰腺、肠道和胃窦黏膜分离的总RNA进行S1核酸酶图谱分析，我们研究了这些组织中胃泌素基因的表达。我们的结果表明，胃泌素基因仅在胃窦黏膜和垂体组织中表达。根据在给定重量的总RNA存在下免受S1核酸酶消化的胃泌素特异性探针的量，垂体中存在的胃泌素mRNA量比胃窦黏膜中低约330倍。这些发现有助于确定胃泌素基因表达的组织分布。
