Qi Yuying, Liu Yanan, Zhang Zixin, Gao Jiaojiao, Guan Zhiyong, Fang Weimin, Chen Sumei, Chen Fadi, Jiang Jiafu
College of Horticulture, Key Laboratory of Landscaping, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.
Hortic Res. 2018 Jul 1;5:37. doi: 10.1038/s41438-018-0037-y. eCollection 2018.
The enzyme RNAPII CTD phosphatase-like 1 is known as a transcriptional regulator of the plant response to various abiotic stresses. Here, the isolation of , a chrysanthemum () gene encoding this enzyme is described. Its predicted 955 residue gene product includes the FCPH catalytic domain, two double-stranded RNA binding motifs, and a nuclear localization signal. A sub-cellular localization assay confirmed that CmCPL1 was expressed in the nucleus. transcription was shown to be significantly inducible by heat stress. The over-expression and knockdown of , respectively, increased and diminished the tolerance of chrysanthemum to heat stress, which maybe dependent on the regulation of CmCPL1 and on the expression of downstream heat stress-responsive genes.
酶RNAPII CTD磷酸酶样1被认为是植物对各种非生物胁迫响应的转录调节因子。本文描述了菊花（）中编码该酶的基因的分离。其预测的955个残基的基因产物包括FCPH催化结构域、两个双链RNA结合基序和一个核定位信号。亚细胞定位分析证实CmCPL1在细胞核中表达。转录显示受热胁迫显著诱导。分别对的过表达和敲低增加和降低了菊花对热胁迫的耐受性，这可能依赖于CmCPL1的调节以及下游热胁迫响应基因的表达。
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