Wang Wen-Jun, Jin Wei, Yang An-Huai, Chen Zhen, Xing Yi-Qiao
Eye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
Int J Ophthalmol. 2018 Jun 18;11(6):923-928. doi: 10.18240/ijo.2018.06.05. eCollection 2018.
To explore the effect of ciliary neurotrophic factor (CNTF) on retinal ganglion cell (RGC)-5 induced by hydrogen peroxide (HO).
After cell adherence, RGC-5 culture medium was changed to contain different concentrations of HO from 50 to 150 µmol/L at four time points (0.5, 1, 1.5 and 2h) to select the concentration and time point for HO induced model. Two different ways of interventions for injured RGC-5 cells respectively were CNTF as an addition in the culture medium or recombinant lentiviral plasmid carrying CNTF gene transfecting bone mesenchymal stem cells (BMSCs) for co-culture with RGC-5.
Compared to the control group, HO led to RGC-5 death closely associated with concentrations and action time of HO and we chose 125 µmol/L and 2h to establish the HO-induced model. While CNTF inhibited the loss of RGC-5 cells obviously with a dose-dependent survival rate. Nevertheless two administration routes had different survival rate yet higher rate in recombinant lentiviral plasmid group but there were no statistically significant differences.
Both the two administration routes of CNTF have effects on RGC-5 cells induced by HO. If their own advantages were combined, there may be a better administration route.
探讨睫状神经营养因子（CNTF）对过氧化氢（HO）诱导的视网膜神经节细胞（RGC）-5的影响。
细胞贴壁后，在四个时间点（0.5、1、1.5和2小时）将RGC-5培养基换成含有50至150μmol/L不同浓度HO的培养基，以选择HO诱导模型的浓度和时间点。分别采用两种不同的干预方式处理受损的RGC-5细胞，一种是在培养基中添加CNTF，另一种是用携带CNTF基因的重组慢病毒质粒转染骨髓间充质干细胞（BMSCs）与RGC-5共培养。
与对照组相比，HO导致RGC-5死亡，且与HO的浓度和作用时间密切相关，我们选择125μmol/L和2小时建立HO诱导模型。而CNTF能明显抑制RGC-5细胞的丢失，其存活率呈剂量依赖性。然而，两种给药途径的存活率不同，重组慢病毒质粒组的存活率较高，但差异无统计学意义。
CNTF的两种给药途径均对HO诱导的RGC-5细胞有影响。若将它们各自的优势结合起来，可能会有更好的给药途径。
