Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide.

AIM

To explore the effect of ciliary neurotrophic factor (CNTF) on retinal ganglion cell (RGC)-5 induced by hydrogen peroxide (HO).

METHODS

After cell adherence, RGC-5 culture medium was changed to contain different concentrations of HO from 50 to 150 µmol/L at four time points (0.5, 1, 1.5 and 2h) to select the concentration and time point for HO induced model. Two different ways of interventions for injured RGC-5 cells respectively were CNTF as an addition in the culture medium or recombinant lentiviral plasmid carrying CNTF gene transfecting bone mesenchymal stem cells (BMSCs) for co-culture with RGC-5.

RESULTS

Compared to the control group, HO led to RGC-5 death closely associated with concentrations and action time of HO and we chose 125 µmol/L and 2h to establish the HO-induced model. While CNTF inhibited the loss of RGC-5 cells obviously with a dose-dependent survival rate. Nevertheless two administration routes had different survival rate yet higher rate in recombinant lentiviral plasmid group but there were no statistically significant differences.

CONCLUSION

Both the two administration routes of CNTF have effects on RGC-5 cells induced by HO. If their own advantages were combined, there may be a better administration route.