Wu Tao, Zhang Xu, Tian Mi, Tao Qiangqiang, Zhang Liang, Ding Yueyun, Zhang Xiaodong, Yin Zongjun
Key Laboratory of Local Animal Genetic Resources Conservation and Bio-breeding of Anhui province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province, People's Republic of China.
J Appl Genet. 2018 Nov;59(4):475-483. doi: 10.1007/s13353-018-0454-5. Epub 2018 Jul 6.
Splay leg is frequently observed in newborn piglets and leads to economic loss as well as welfare concerns. However, the etiology and pathogenesis of splay leg syndrome in piglets are still poorly understood. The aims of this paper were to characterize changes in the transcriptome of splay leg piglets and identify candidate genes responsible for this disease. We chose three splay leg piglets and their healthy full sibs, and constructed six RNA libraries using skeletal muscle samples from both groups and identified the differentially expressed genes between the two groups using RNA-seq. A total of 555 differentially expressed genes were identified, of which 216 were up-regulated and 339 genes were down-regulated in the splay leg group relative to the healthy group. In addition, 321 significantly enriched GO terms and 12 significantly enriched KEGG pathways were identified. FBXO32 is one of the ten most differentially expressed genes in our experiment, and it is regulated by the significantly enriched pathway (PI3K-Akt). The overexpression of FBXO32 which leads to the process of muscle atrophy might be responsible for congenital splay leg in piglets. The result of this study could help improve understanding of the molecular mechanism of congenital splay leg syndrome.
仔猪八字腿在新生仔猪中经常出现，会导致经济损失以及引发福利问题。然而，仔猪八字腿综合征的病因和发病机制仍未得到充分了解。本文的目的是表征八字腿仔猪转录组的变化，并确定导致这种疾病的候选基因。我们选择了三只八字腿仔猪及其健康的同窝全同胞，使用两组的骨骼肌样本构建了六个RNA文库，并通过RNA测序确定两组之间的差异表达基因。共鉴定出555个差异表达基因，其中相对于健康组，八字腿组中有216个基因上调，339个基因下调。此外，还鉴定出321个显著富集的GO术语和12条显著富集的KEGG途径。FBXO32是我们实验中差异表达最显著的十个基因之一，它受显著富集的途径（PI3K-Akt）调控。FBXO32的过表达导致肌肉萎缩过程，可能是仔猪先天性八字腿的原因。本研究结果有助于提高对先天性八字腿综合征分子机制的理解。
