Department of Mechanical Engineering, Sogang University, 35 Baekbeom-ro (Shinsu-dong), Mapo-gu, Seoul 04107, South Korea.
Lab Chip. 2018 Jul 24;18(15):2270-2278. doi: 10.1039/c8lc00227d.
In this study, a new strategy for improving the radionuclide bio-decontamination (RBD) activity of microalgae by screening a better strain with high potential for biomineral production has been proposed. A noninvasive dielectrophoresis (DEP)-based microalgae screening microplatform has been used to select the highly capable microalgae in RBD. Microalgae (Chlorella vulgaris KMMCC9) with a high degree of competence in strontium (Sr) removal were successfully segregated against Chlorella vulgaris KCTC AG10002 that has relatively weak Sr removal activity under an AC electric field. C. vulgaris KMMCC9 with higher Sr biomineral competence (HSC) was also successfully segregated against others with lower Sr biomineral competence (LSC). Furthermore, after the screening and large-scale cultivation of C. vulgaris KMMCC9 with HSC, the microalgae showed highly effective Sr bio-decontamination in both non-radioactive and radioactive Sr contaminated water compared to wild-type (WT).
在这项研究中，提出了一种通过筛选具有高生物矿化潜力的更好菌株来提高微藻放射性核素生物去污（RBD）活性的新策略。使用基于非侵入性介电泳（DEP）的微藻筛选微平台来选择在 RBD 中具有高能力的微藻。在交流电场下，成功地将具有高锶（Sr）去除能力的微藻（普通小球藻 KMMCC9）与相对较弱 Sr 去除活性的普通小球藻 KCTC AG10002 分离。还成功地将具有更高 Sr 生物矿化能力（HSC）的普通小球藻 KMMCC9 与其他 Sr 生物矿化能力较低的微藻（LSC）分离。此外，在对 HSC 的普通小球藻 KMMCC9 进行筛选和大规模培养后，与野生型（WT）相比，该微藻在非放射性和放射性 Sr 污染水中表现出高度有效的 Sr 生物去污能力。
