Yan Yan, Li Qiang, Du Chen-hui, Jia Jin-ping, Feng Hong-xia, Qin Xue-mei
Yao Xue Xue Bao. 2017 Feb;52(2):283-90.
The study aims to investigate the effective components of Semen Ziziphi Spinosae (SZR) in nourishing the heart and tranquilizing the mind. A method of ultra high liquid chromatography (UHPLC) coupled with Q Exactive high resolution mass spectrometry (HR-MS) was developed. Based on the UV spectra, retention time and MS spectra, 25 compounds of SZR extract were identified or tentatively characterized, including 12 flavonoids, 8 triterpenoids saponins, 2 fatty acid and 3 alakoids. The study illuminated the major chemical components. Twenty bioactive components were determined in rat urine after oral administration of SZR extract by “in vitro to in vivo” translation approach, including 16 prototype compounds and 4 metabolites. Spinosin, swertisin, jujuboside A and B were considered as the effective and active constituents in SZR of the sedative and hypnotic effects, which emodies characteristics of multiple components. It was beneficial exploration for searching the effective and active constituents of SZR in nourishing the heart and tranquilizing the mind.
本研究旨在探讨酸枣仁养心安神的有效成分。建立了超高效液相色谱（UHPLC）与Q Exactive高分辨率质谱（HR-MS）联用的方法。根据紫外光谱、保留时间和质谱，鉴定或初步表征了酸枣仁提取物中的25种化合物，包括12种黄酮类、8种三萜皂苷、2种脂肪酸和3种生物碱。该研究阐明了主要化学成分。通过“体外到体内”的转化方法，在大鼠口服酸枣仁提取物后的尿液中测定了20种生物活性成分，包括16种原型化合物和4种代谢产物。斯皮诺素、獐牙菜苷、酸枣仁皂苷A和B被认为是酸枣仁镇静催眠作用的有效活性成分，体现了多成分的特点。这为寻找酸枣仁养心安神的有效活性成分提供了有益的探索。
