Institute of Crop Science and Resource Conservation - Horticultural Science, University of Bonn, Auf dem Huegel 6, 53121, Bonn, Germany.
Institute of Crop Science and Resource Conservation - Horticultural Science, University of Bonn, Auf dem Huegel 6, 53121, Bonn, Germany.
Plant Physiol Biochem. 2018 Sep;130:105-111. doi: 10.1016/j.plaphy.2018.06.033. Epub 2018 Jun 27.
Agricultural residues are natural sources for secondary metabolites as high value ingredients for industrial uses. The present work aims to exploit the accumulation potential of rutin and solanesol in tomato leaves following nitrogen and general mineral deficiency in a commercial-like greenhouse. Physiological responses of tomato plants were monitored non-destructively with a multiparametric fluorescence sensor, and biochemical parameters were determined by means of HPLC analysis. Nitrogen and general mineral limitation led to an accumulation of rutin in young tomato leaves while solanesol concentration was higher in mature leaves. In young leaves, the fluorescence indices SFR_R and NBI_G showed lower values compared to control plants for both stress treatments. On the contrary, FLAV and ANTH_RG values increased during the experiment, but no differences could be recorded in mature leaves. However, correlation analysis indicates, that the FLAV index is not a reliable tool to estimate the concentration of rutin and solanesol tomato leaves. To monitor fruit yield/quality as primary objective of tomato production, fruits showing symptoms of blossom end rot (BER) were counted before and after stress treatments. BER was determined more frequently for plants grown under a general mineral deficiency, concluding that a practical applicability at the end of fruit production is advisable. Our results indicate that by-products from Solanaceae plants are promising resources for valuable bioactive leaf compounds. To achieve the highest concentrations, the seasonal variation, the optimal environmental conditions, the concentrations in different plant organs and varieties as well as different production systems are of high interest for commercial implementation.
农业残余物是次生代谢物的天然来源，可作为工业用途的高价值成分。本工作旨在探索番茄叶片中芦丁和茄呢醇在商业温室中氮和一般矿物质缺乏条件下的积累潜力。使用多参数荧光传感器对番茄植株的生理反应进行非破坏性监测，并通过 HPLC 分析测定生化参数。氮和一般矿物质限制导致幼叶中芦丁的积累，而茄呢醇浓度在成熟叶片中较高。在年轻的番茄叶片中，与对照植物相比，两种胁迫处理的 SFR_R 和 NBI_G 荧光指数值较低。相反，FLAV 和 ANTH_RG 值在实验过程中增加，但在成熟叶片中未记录到差异。然而，相关分析表明，FLAV 指数不是估计番茄叶片中芦丁和茄呢醇浓度的可靠工具。为了监测果实产量/质量作为番茄生产的主要目标，在胁迫处理前后统计出现花端腐烂 (BER) 症状的果实。在一般矿物质缺乏的情况下，植物出现 BER 的频率更高，这表明在果实生产结束时具有实际应用的可行性。我们的结果表明，茄科植物的副产品是有前途的有价值生物活性叶化合物资源。为了达到最高浓度，季节性变化、最佳环境条件、不同植物器官和品种中的浓度以及不同的生产系统是商业实施的高度关注。
