Azad A A, Barrett S A, Fahey K J
Virology. 1985 May;143(1):35-44. doi: 10.1016/0042-6822(85)90094-7.
The genome of infectious bursal disease virus (IBDV) strain 002-73 was found to consist of two segments of double-stranded (ds) RNA which were 3400 bp (MW 2.06 X 10(6)) and 2900 bp (MW 1.76 X 10(6)) long, respectively. The ds IBDV RNA could be translated, in vitro, only after extensive denaturation. The small RNA segment was found to code for a single polypeptide of MW 90K, while the large RNA segment coded for three major polypeptides of MW 52K, 32K, and 28K, and two minor polypeptides of MW 41K and 16K. The large RNA segment could encode proteins of MW 125K while the MW of the translated products was 169K suggesting that a precursor-product relationship exists between some of the translation products. A method is described for the synthesis of ds cDNA from large ds RNA molecules. Analyses of recombinant colonies showed that inserts covering the entire IBDV genome had been cloned.
传染性法氏囊病病毒（IBDV）002 - 73株的基因组由两段双链（ds）RNA组成，长度分别为3400 bp（分子量2.06×10⁶）和2900 bp（分子量1.76×10⁶）。ds IBDV RNA只有在经过广泛变性后才能在体外进行翻译。发现小RNA片段编码一种分子量为90K的单一多肽，而大RNA片段编码三种主要多肽，分子量分别为52K、32K和28K，以及两种次要多肽，分子量分别为41K和16K。大RNA片段可编码分子量为125K的蛋白质，而翻译产物的分子量为169K，这表明一些翻译产物之间存在前体 - 产物关系。描述了一种从大ds RNA分子合成ds cDNA的方法。重组菌落分析表明，已克隆出覆盖整个IBDV基因组的插入片段。
