对小鼠毒力高低不同的小鼠肝炎病毒对干扰素敏感性的差异。
Difference in sensitivity to interferon among mouse hepatitis viruses with high and low virulence for mice.
作者信息
Taguchi F, Siddell S G
出版信息
Virology. 1985 Nov;147(1):41-8. doi: 10.1016/0042-6822(85)90225-9.
Abstract
Mouse hepatitis viruses (MHV) of different virulence for mice were studied with respect to interferon (IFN) sensitivity. The growth of low-virulent MHV-S and intermediately virulent MHV-JHM was significantly suppressed in IFN-treated L cells compared with untreated cells. However, a comparable suppression of the growth of highly virulent MHV-2 was not observed in IFN-treated cells. This differential effect of IFN treatment could also be demonstrated at the level of viral mRNA and viral proteins. In cells infected with MHV-S or MHV-JHM the amount of viral mRNAs was remarkably reduced by IFN treatment. Also the levels of the major intracellular viral proteins, in particular the E1 protein, were affected by IFN treatment. Similar effects could not be demonstrated in MHV-2-infected cells. These results suggest that during MHV-S or MHV-JHM infection IFN treatment suppresses virus replication at several stages. The significance of these results is discussed in terms of the pathogenecity of these viruses.
摘要
针对小鼠不同毒力的小鼠肝炎病毒（MHV）对干扰素（IFN）的敏感性进行了研究。与未处理的细胞相比，在经干扰素处理的L细胞中，低毒力的MHV-S和中等毒力的MHV-JHM的生长受到显著抑制。然而，在经干扰素处理的细胞中未观察到高毒力的MHV-2生长受到类似抑制。干扰素处理的这种差异效应在病毒mRNA和病毒蛋白水平也得到了证实。在用MHV-S或MHV-JHM感染的细胞中，干扰素处理显著减少了病毒mRNA的量。主要细胞内病毒蛋白的水平，特别是E1蛋白的水平，也受到干扰素处理的影响。在感染MHV-2的细胞中未观察到类似效应。这些结果表明，在MHV-S或MHV-JHM感染期间，干扰素处理在多个阶段抑制病毒复制。根据这些病毒的致病性对这些结果的意义进行了讨论。
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本文引用的文献
1
MOUSE MACROPHAGES AS HOST CELLS FOR THE MOUSE HEPATITIS VIRUS AND THE GENETIC BASIS OF THEIR SUSCEPTIBILITY.小鼠巨噬细胞作为小鼠肝炎病毒的宿主细胞及其易感性的遗传基础
Proc Natl Acad Sci U S A. 1960 Aug;46(8):1065-75. doi: 10.1073/pnas.46.8.1065.
2
Coronavirus JHM: cell-free synthesis of structural protein p60.冠状病毒JHM：结构蛋白p60的无细胞合成
J Virol. 1980 Jan;33(1):10-7. doi: 10.1128/JVI.33.1.10-17.1980.
3
Coronavirus JHM: intracellular protein synthesis.冠状病毒JHM：细胞内蛋白质合成
J Gen Virol. 1981 Mar;53(Pt 1):145-55. doi: 10.1099/0022-1317-53-1-145.
4
The molecular biology of coronaviruses.冠状病毒的分子生物学
Adv Virus Res. 1983;28:35-112. doi: 10.1016/s0065-3527(08)60721-6.
5
Coronavirus JHM: nucleotide sequence of the mRNA that encodes nucleocapsid protein.冠状病毒JHM：编码核衣壳蛋白的mRNA的核苷酸序列。
Nucleic Acids Res. 1983 Aug 11;11(15):5045-54. doi: 10.1093/nar/11.15.5045.
6
The biology of coronaviruses.冠状病毒的生物学特性。
J Gen Virol. 1983 Apr;64 (Pt 4):761-76. doi: 10.1099/0022-1317-64-4-761.
7
Replication of mouse hepatitis viruses with high and low virulence in cultured hepatocytes.高毒力和低毒力小鼠肝炎病毒在培养的肝细胞中的复制
Infect Immun. 1983 Feb;39(2):955-9. doi: 10.1128/iai.39.2.955-959.1983.
8
Replication of mouse hepatitis virus: negative-stranded RNA and replicative form RNA are of genome length.小鼠肝炎病毒的复制：负链RNA和复制型RNA具有基因组长度。
J Virol. 1982 Nov;44(2):487-92. doi: 10.1128/JVI.44.2.487-492.1982.
9
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10
Comparison of mouse hepatitis virus strains for pathogenicity in weanling mice infected by various routes.通过不同途径感染断奶小鼠的情况下，小鼠肝炎病毒毒株致病性的比较。
Arch Virol. 1981;70(1):69-73. doi: 10.1007/BF01320795.
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