Fried G, Hammarström M, Dahlin I
Acta Physiol Scand. 1985 Mar;123(3):311-6. doi: 10.1111/j.1748-1716.1985.tb07593.x.
A noradrenaline (NA) storage vesicle fraction was isolated from the uteri of virgin as well as pregnant guinea-pigs. The uptake of 3H-NA into respective vesicle fractions was compared. Total uptake of 3H-NA in the presence of Mg2+ (2.5mM) and ATP (2.5 mM) at 30 degrees C after 20 min was 6.0 +/- 1.4 pmol . mg-1 in uterus fractions from virgin guinea-pigs as compared to 1.87 +/- 0.41 pmol . mg-1 in uterus fractions from pregnant animals. When desipramine (5mM) was added in order to block neuronal uptake the corresponding figures were 4.75 +/- 0.92 and 0.51 +/- 0.18 pmol . mg-1, respectively. Thus in the presence of desipramine (5mM) uptake was reduced by 21.4% in fractions from virgin uteri but by 72.7% in fractions from pregnant uteri. Reserpine (0.1 microM) inhibited uptake of 3H-NA by 34% in fractions from virgin uteri and by 46% in fractions from pregnant uteri. Our results indicate a decreased amount of functional storage vesicles in uteri of guinea-pigs at term pregnancy and are consistent with the proposed degeneration of adrenergic nerves during pregnancy.
从未孕和妊娠豚鼠的子宫中分离出去甲肾上腺素（NA）储存囊泡组分。比较了3H-NA在各囊泡组分中的摄取情况。在30℃下，20分钟后，在存在Mg2 +（2.5mM）和ATP（2.5mM）的情况下，未孕豚鼠子宫组分中3H-NA的总摄取量为6.0±1.4 pmol·mg-1，而妊娠动物子宫组分中为1.87±0.41 pmol·mg-1。当加入地昔帕明（5mM）以阻断神经元摄取时，相应的数据分别为4.75±0.92和0.51±0.18 pmol·mg-1。因此，在存在地昔帕明（5mM）的情况下，未孕子宫组分中的摄取减少了21.4％，而妊娠子宫组分中的摄取减少了72.7％。利血平（0.1μM）抑制未孕子宫组分中3H-NA摄取的34％，抑制妊娠子宫组分中3H-NA摄取的46％。我们的结果表明，足月妊娠豚鼠子宫中功能性储存囊泡的数量减少，这与怀孕期间拟交感神经的退化一致。
