一种用于人体壁细胞酸生成体外研究的方法。组胺、五肽胃泌素和卡巴胆碱的刺激作用。

A method for in vitro studies on acid formation in human parietal cells. Stimulation by histamine, pentagastrin and carbachol.

作者信息

Mårdh S, Norberg L, Ljungström M, Wollert S, Nyrén O, Gustavsson S

出版信息

Acta Physiol Scand. 1985 Mar;123(3):349-54. doi: 10.1111/j.1748-1716.1985.tb07599.x.

DOI:10.1111/j.1748-1716.1985.tb07599.x

Abstract

Cells were isolated from human gastric mucosa on a large scale from gastric resection specimens and on a microscale from endoscopic biopsies by sequential incubations with pronase and collagenase. The accumulation of aminopyrine (AP) was used as an index of acid production in the parietal cells. Basal accumulation was about 0.2 pmol AP/10(4) parietal cells. Addition of histamine, db-cAMP, pentagastrin and carbachol increased the aminopyrine accumulation. Maximal accumulation was of the order of 1000-2800% of the control and was obtained after stimulation by 10(-4) M histamine and by 10(-3) M db-cAMP. Stimulation by pentagastrin and by carbachol reached 200 to 350% of the control. EC50 was 2 X 10(-6) M for histamine, 10(-8) M for pentagastrin, and 4 X 10(-6) M for carbachol. Human parietal cells were enriched from a mixture of gastric mucosal cells by isopycnic centrifugation on density gradients of Percoll. A parietal cell fraction with a purity of 83% was obtained. The density of human parietal cells was estimated to 1.06 g . ml-1.

摘要

通过用链霉蛋白酶和胶原酶依次孵育，从胃切除标本中大规模分离人胃黏膜细胞，并从内镜活检标本中微量分离。氨基比林（AP）的积累用作壁细胞产酸的指标。基础积累约为0.2 pmol AP/10⁴个壁细胞。添加组胺、二丁酰环磷腺苷（db-cAMP）、五肽胃泌素和卡巴胆碱可增加氨基比林的积累。最大积累量为对照的1000 - 2800%左右，在10⁻⁴ M组胺和10⁻³ M db-cAMP刺激后获得。五肽胃泌素和卡巴胆碱刺激达到对照的200%至350%。组胺的半数有效浓度（EC50）为2×10⁻⁶ M，五肽胃泌素为10⁻⁸ M，卡巴胆碱为4×10⁻⁶ M。通过在Percoll密度梯度上进行等密度离心，从胃黏膜细胞混合物中富集人壁细胞。获得了纯度为83%的壁细胞组分。人壁细胞的密度估计为1.06 g·ml⁻¹。