Popov I L, Baraĭ V N, Zinchenko A I, Chernov S P, Kvasiuk E I
Antibiot Med Biotekhnol. 1985 Aug;30(8):588-91.
Strain 47/3 was isolated from the natural sources of Erwinia herbicola. The cells of this strain contain nucleoside phosphate transferase responsible for specific phosphorylation of the adenosine 5'-hydroxyl group. With the use of the strain intact cells, conditions for preparation of AMP were determined. The cells were grown in the meat-peptone broth supplemented with yeast extract. p-Nitrophenylphosphate was used as a donor of phosphate groups. It was shown that the concentration of the reaction substrates, their ratio and PH of the reaction mixture were factors defining the cell activity. Under the optimal conditions (0.2 M acetate buffer, pH 4.5; concentrations of p-nitrophenylphosphate and adenosine 96 and 12 mg/ml respectively; concentration of the cells 1 per cent) the cells phosphorylated adenosine with a yield of 74 mol %. This indicates that the method may be used for production of AMP in preparative amounts.
菌株47/3是从草本欧文氏菌的天然来源中分离得到的。该菌株的细胞含有负责腺苷5'-羟基特异性磷酸化的核苷磷酸转移酶。利用该菌株的完整细胞，确定了制备AMP的条件。细胞在添加酵母提取物的肉蛋白胨肉汤中生长。对硝基苯磷酸酯用作磷酸基团供体。结果表明，反应底物的浓度、它们的比例以及反应混合物的pH值是决定细胞活性的因素。在最佳条件下（0.2M醋酸盐缓冲液，pH4.5；对硝基苯磷酸酯和腺苷的浓度分别为96和12mg/ml；细胞浓度为1%），细胞使腺苷磷酸化，产率为74mol%。这表明该方法可用于制备大量的AMP。
