Raval P J, Allan D
Biochem J. 1985 Oct 1;231(1):179-83. doi: 10.1042/bj2310179.
Treatment of chicken erythrocytes with ionophore A23187 and Ca2+ caused the breakdown of a large proportion of the cellular polyphosphoinositides. Since no diacylglycerol or phosphatidate was generated, but there was a small increase in the level of phosphatidylinositol, it was concluded that breakdown occurred as a result of phosphomonoesterase activation. Experiments with subcellular fractions showed that the phosphomonoesterase activity was present in the cytosolic fraction of the cells.
用离子载体A23187和Ca2+处理鸡红细胞,导致细胞中大部分多磷酸肌醇分解。由于未生成二酰基甘油或磷脂酸,但磷脂酰肌醇水平略有升高,因此得出结论,分解是磷酸单酯酶激活的结果。亚细胞组分实验表明,磷酸单酯酶活性存在于细胞的胞质组分中。