通过基质金属蛋白酶（MMP）表达的分子成像预测替莫唑胺的抗胶质瘤治疗效果。
Prediction of the anti-glioma therapeutic effects of temozolomide through molecular imaging of MMP expression.
作者信息
Li Li, Du Yang, Xiang Dehui, Chen Liang, Shi Zhifeng, Tian Jie, Chen Xinjian
机构信息
School of Electronic and Information Engineering, Soochow University, No. 1 Ten Azusa Street, Suzhou, 215006, China.
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, 215123, China.
出版信息
Biomed Opt Express. 2018 Jun 20;9(7):3193-3207. doi: 10.1364/BOE.9.003193. eCollection 2018 Jul 1.
Currently, there is no effective way to assess the therapeutic response of temozolomide (TMZ) for the glioma. In this study, the human U87MG-fLuc glioma animal models were set up and the antitumor efficacy of TMZ was evaluated using bioluminescence imaging (BLI) and MRI. Then, bioluminescence tomography (BLT) was reconstructed using an adaptive sparsity matching pursuit (ASMP) algorithm. Second, the expression level of the MMP-750 probe was examined with or without TMZ treatment using FMI. Third, the expression of MMP2 and MMP3 was specifically examined after treatment. The results showed that TMZ effectively inhibited glioma growth. The targeted imaging of MMP-750 was decreased during the treatment of glioma with TMZ. Moreover, the MMP2 and MMP3 expression was found to correlate with the inhibition effect of TMZ. Our study indicated that the therapeutic effects of TMZ can be effectively evaluated at an early stage using molecular imaging, and MMP targeting the fluorescence probe could be utilized for the prediction and assessment of the therapeutic effects of TMZ.
目前，尚无有效的方法来评估替莫唑胺（TMZ）对胶质瘤的治疗反应。在本研究中，建立了人U87MG-fLuc胶质瘤动物模型，并使用生物发光成像（BLI）和磁共振成像（MRI）评估了TMZ的抗肿瘤疗效。然后，使用自适应稀疏匹配追踪（ASMP）算法重建生物发光断层扫描（BLT）。其次，使用荧光分子成像（FMI）检测有无TMZ治疗时MMP-750探针的表达水平。第三，在治疗后特异性检测MMP2和MMP3的表达。结果显示，TMZ有效抑制了胶质瘤生长。在用TMZ治疗胶质瘤期间，MMP-750的靶向成像降低。此外，发现MMP2和MMP3的表达与TMZ的抑制作用相关。我们的研究表明，使用分子成像可在早期有效评估TMZ的治疗效果，且靶向MMP的荧光探针可用于预测和评估TMZ的治疗效果。
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