Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Department of Parasitology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran.
Biomed Res Int. 2018 Jun 6;2018:6049198. doi: 10.1155/2018/6049198. eCollection 2018.
Iran is one of the six countries with the most cutaneous leishmaniasis (CL) patients. Understanding better the genotypes of the parasite population in relation to geography and climate is critical to achieving better CL control. We aimed to characterise the population structure of , the cause of anthroponotic cutaneous leishmaniasis (ACL), from important foci in southeast (Bam and Kerman) and southwest (Shiraz) Iran. A total of 39 isolates from ACL patients from southeast (Bam 14, Kerman 12) and southwest (Shiraz 13) Iran were analysed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) of the kinetoplast DNA (kDNA) using restriction enzymes I () and I. 37 genotypes were identified among south Iran isolates. The unweighted pair group method with arithmetic mean (UPGMA) tree obtained from the banding patterns of I digested kDNA RFLP distinguished southeast from and southwest isolates with some subclustering but the I derived tree showed greater discrimination with greater subclustering and divergence of the two foci of southeast region but with some overlapping. Although a monophyletic structure has been defined for southeast , isolates from two foci of southeast Iran were partly discriminated in the current study.
伊朗是皮肤利什曼病（CL）患者最多的六个国家之一。了解寄生虫种群的基因型与地理和气候的关系对于实现更好的 CL 控制至关重要。我们旨在描述引起人间皮肤利什曼病（ACL）的的种群结构，该寄生虫主要集中在伊朗东南部（Bam 和克尔曼）和西南部（设拉子）。使用限制性内切酶 I（）和 I 对来自伊朗东南部（Bam 14，克尔曼 12）和西南部（设拉子 13）的 ACL 患者的 39 株分离株进行了聚合酶链反应限制性片段长度多态性（PCR-RFLP）分析。对南伊朗的 37 个 分离株进行了分析。未加权对组平均（UPGMA）树来自 I 消化的 kDNA RFLP 的带型区分了东南部和西南部的 分离株，但 I 衍生的树显示出更大的区分度，具有更大的聚类和东南地区两个焦点的发散，但有一些重叠。尽管已经为东南部定义了一个单系结构，但本研究部分区分了伊朗东南部两个焦点的分离株。
