锌指蛋白和干扰素刺激基因在艾滋病患者接受高效抗逆转录病毒治疗后的结核免疫重建炎症综合征中起着至关重要的作用。

Zinc finger and interferon-stimulated genes play a vital role in TB-IRIS following HAART in AIDS.

作者信息

Ma Jinmin, Zhao Fang, Su Wei, Li Qiongfang, Li Jiandong, Ji Jingkai, Deng Yong, Zhou Yang, Wang Xinfa, Yang Huanming, Saksena Nitin K, Kristiansen Karsten, Wang Hui, Liu Yingxia

机构信息

BGI-Shenzhen, Shenzhen 518083, PR China.

Laboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, Copenhagen 2100, Denmark.

出版信息

Per Med. 2018 Jul 1;15(4):251-269. doi: 10.2217/pme-2017-0084. Epub 2018 Jul 9.

DOI:10.2217/pme-2017-0084

PMID:29984631

Abstract

AIM

Co-infection in HIV-1 patients with Mycobacterium tuberculosis poses considerable risk of developing the immune reconstitution inflammatory syndrome (IRIS), especially upon the initiation of antiretroviral therapy (ART). Methodology & results: For transcriptomic analysis, peripheral blood mononuclear cells' whole gene expression was used from three patient groups: HIV (H), HIV-TB (HT), HIV-TB with IRIS (HTI). Pathway enrichment and functional analysis was performed before and after highly active ART. Genes in the interferon-stimulating and ZNF families maintained tight functional interaction and tilted the balance in favor of TB-IRIS.

DISCUSSION & CONCLUSION: The functional impairment of interaction between ZNF genes and interferon-stimulated genes, along with higher expression of S100A8/S100A9 genes possibly forms the genomic basis of TB-IRIS in a subset of HIV patients while on highly active ART.

摘要

目的

HIV-1患者合并结核分枝杆菌感染有发生免疫重建炎症综合征（IRIS）的重大风险，尤其是在开始抗逆转录病毒治疗（ART）时。方法与结果：为进行转录组分析，使用了三个患者组外周血单个核细胞的全基因表达：HIV组（H）、HIV-结核组（HT）、伴有IRIS的HIV-结核组（HTI）。在高效ART前后进行了通路富集和功能分析。干扰素刺激基因家族和锌指蛋白（ZNF）家族中的基因保持紧密的功能相互作用，并使平衡向有利于结核-IRIS的方向倾斜。