核酸酶S1在DNA异源双链体中的两碱基缺失错配处产生双链切割。
Double-strand cleavage at a two-base deletion mismatch in a DNA heteroduplex by nuclease S1.
作者信息
Burdon M G, Lees J H
出版信息
Biosci Rep. 1985 Aug;5(8):627-32. doi: 10.1007/BF01116993.
PMID:2998501
Abstract
A two-base deletion mismatch was generated in a DNA heteroduplex by hybridization of two linear plasmid DNA molecules differing only by the presence of a two-base deletion in one of them. The heteroduplex was shown to be sensitive to double-strand cleavage by nuclease S1, thus demonstrating the potential value of single-stranded probes for the detection of polymorphisms in genomic DNA due to very small deletions.
摘要
通过两个仅在其中一个存在两碱基缺失的线性质粒DNA分子杂交，在DNA异源双链体中产生了两碱基缺失错配。该异源双链体对核酸酶S1的双链切割敏感，从而证明了单链探针对于检测由于非常小的缺失而导致的基因组DNA多态性的潜在价值。
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