Burdon M G, Lees J H
Biosci Rep. 1985 Aug;5(8):627-32. doi: 10.1007/BF01116993.
A two-base deletion mismatch was generated in a DNA heteroduplex by hybridization of two linear plasmid DNA molecules differing only by the presence of a two-base deletion in one of them. The heteroduplex was shown to be sensitive to double-strand cleavage by nuclease S1, thus demonstrating the potential value of single-stranded probes for the detection of polymorphisms in genomic DNA due to very small deletions.
通过两个仅在其中一个存在两碱基缺失的线性质粒DNA分子杂交，在DNA异源双链体中产生了两碱基缺失错配。该异源双链体对核酸酶S1的双链切割敏感，从而证明了单链探针对于检测由于非常小的缺失而导致的基因组DNA多态性的潜在价值。
