Ruiz-Carrillo A
Nucleic Acids Res. 1984 Aug 24;12(16):6473-92. doi: 10.1093/nar/12.16.6473.
The potential of the cloned histone H5 gene to form altered DNA structures has been examined by S1 nuclease digestion of supercoiled recombinant plasmids containing up to 8.8 kbp of chicken DNa. The three main nicking sites map at the upstream and downstream sequences flanking the structural gene. The cleavage sites share sequence homology, strand specificity, and do not seem to be single-stranded. The sequence of the S1-sensitive sites does not suggest that the fragments can adopt any of the known DNA secondary structures.
通过对含有长达8.8kbp鸡DNA的超螺旋重组质粒进行S1核酸酶消化，研究了克隆的组蛋白H5基因形成改变的DNA结构的潜力。三个主要的切口位点位于结构基因两侧的上游和下游序列处。切割位点具有序列同源性、链特异性，且似乎不是单链的。S1敏感位点的序列并不表明这些片段可以采用任何已知的DNA二级结构。
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