Department of Chemistry, School of Science, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Center of Excellence for Innovation in Chemistry (PERCH-CIC), Department of Chemistry, Faculty of Science, Mahasarakham University, Kantharawichai District, Mahasarakham 44150, Thailand.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Nov 5;204:726-734. doi: 10.1016/j.saa.2018.06.109. Epub 2018 Jul 3.
The use of natural reagents from plant extracts for chemical analysis is one approach in the development of green analytical chemistry methodology. In this work, a natural reagent extracted from Phyllanthus emblica Linn. has been applied for the determination of iron(III) using a simple flow injection spectrophotometric method. The method was based on the measurement of a dark-purple complex formed by the reaction between iron(III) and the extracted solution in an acetate buffer (pH 5.6) at 570 nm. Under the optimum conditions, a linear calibration graph in the range of 0.50-20.0 mg L iron(III) was obtained with a correlation coefficient (r) of 0.9996. The limit of detection and limit of quantification were 0.31 and 0.50 mg L, respectively. The relative standard deviation was less than 2.50%. The proposed method was successfully applied for quantitative analysis of iron(III) in pharmaceutical preparations and water samples with a sampling rate of 90 samples h. The results are in good agreement with those obtained by the official ICP-OES technique at the 95% confidence level. The presented method provides a simple, cost-effective and environmentally friendly approach which is suitable and useful for determining iron(III). Therefore, it can be considered as an alternative analytical technique in green chemistry.
利用植物提取物中的天然试剂进行化学分析是绿色分析化学方法发展的一种方法。在这项工作中，从余甘子中提取出一种天然试剂，用于采用简单的流动注射分光光度法测定铁（III）。该方法基于在乙酸盐缓冲液（pH 5.6）中，铁（III）与提取液反应形成深紫色络合物，在 570nm 处进行测量。在最佳条件下，获得了 0.50-20.0mg/L 铁（III）范围内的线性校准曲线，相关系数（r）为 0.9996。检测限和定量限分别为 0.31 和 0.50mg/L。相对标准偏差小于 2.50%。该方法成功地用于测定药物制剂和水样中的铁（III），采样速率为 90 个样品/h。在 95%置信水平下，结果与官方 ICP-OES 技术获得的结果吻合良好。所提出的方法提供了一种简单、经济有效且环保的方法，适用于测定铁（III）。因此，可以将其视为绿色化学中替代分析技术。
