Department of Nuclear Medicine, Odense University Hospital, Kløvervænget 47, 5000, Odense, Denmark.
Department of Clinical Research, University of Southern Denmark, Odense, Denmark.
Mol Imaging Biol. 2019 Apr;21(2):375-381. doi: 10.1007/s11307-018-1247-2.
Malignant cells exhibit increased rates of aerobic glycolysis. Here, we tested whether the accumulation of fluoro-deoxyglucose-6-phosphate (FDG6P) in ovarian cancers of differential malignancy reflects inversely correlated elevations of hexokinase (HK) and glucose-6-phosphatase (G6Pase) activities.
Twenty-nine women with suspected ovarian cancer had positron emission tomography (PET) prior to surgery. From fresh-frozen tissue, we determined the activities of HK and G6Pase, and from the PET images, we determined the tumor maximum standardized uptake value (SUVmax) of 2-deoxy-2-[F]fluoro-D-glucose.
The SUVmax of malignant lesions significantly exceeded the SUVmax of benign (p < 0.005) and borderline lesions (p < 0.0005) that did not differ significantly. We found no significant correlation between measured HK or G6Pase activities and histological tumor type or SUVmax except that G6Pase activities were higher in malignant than borderline lesions (p < 0.05). Measured HK and G6Pase activities correlated inversely (p < 0.05). The slopes from the regression lines of the three correlations yielded positively correlated abscissa and ordinate intercepts, designated HK and G6Pase, respectively (r = 0.67, p < 0.0001). The positive correlations between the abscissa and ordinate intercepts with SUVmax had regression coefficients of r = 0.44, p < 0.05; and r = 0.39, p < 0.05, respectively.
The results distinguished two ovarian cancer phenotypes, one with elevated HK activity and low G6Pase activity, and another with the opposite characteristics.
恶性细胞表现出有氧糖酵解率的增加。在这里，我们测试了在不同恶性程度的卵巢癌中，氟代脱氧葡萄糖-6-磷酸（FDG6P）的积累是否反映了己糖激酶（HK）和葡萄糖-6-磷酸酶（G6Pase）活性的反比升高。
29 名疑似卵巢癌的女性在手术前进行了正电子发射断层扫描（PET）。从新鲜冷冻组织中，我们确定了 HK 和 G6Pase 的活性，从 PET 图像中，我们确定了 2-脱氧-2-[F]氟代-D-葡萄糖的肿瘤最大标准化摄取值（SUVmax）。
恶性病变的 SUVmax 明显高于良性（p<0.005）和交界性病变（p<0.0005），且良性和交界性病变之间无显著差异。除了 G6Pase 活性在恶性病变中高于交界性病变（p<0.05）外，我们未发现测量的 HK 或 G6Pase 活性与组织学肿瘤类型或 SUVmax 之间存在显著相关性。测量的 HK 和 G6Pase 活性呈负相关（p<0.05）。三条相关性回归线的斜率产生了正相关的横坐标和纵坐标截距，分别命名为 HK 和 G6Pase（r=0.67，p<0.0001）。横坐标和纵坐标截距与 SUVmax 之间的正相关关系的回归系数分别为 r=0.44，p<0.05；和 r=0.39，p<0.05。
结果区分了两种卵巢癌表型，一种具有升高的 HK 活性和低 G6Pase 活性，另一种具有相反的特征。
