小鼠巨噬细胞中的功能基因组学
Functional Genomics in Murine Macrophages.
作者信息
Lee Frank Fang-Yao, Alper Scott
机构信息
Department of Biomedical Research and Center for Genes, Environment and Health, National Jewish Health, Denver, CO, USA.
Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, USA.
出版信息
Methods Mol Biol. 2018;1809:289-298. doi: 10.1007/978-1-4939-8570-8_18.
Abstract
In this chapter, we describe methods for functional genomics studies in mouse macrophages. In particular, we describe complementary methods for gene inhibition using RNA interference (RNAi) and gene overexpression. These methods are readily amenable to medium- and high-throughput functional genomics investigations. These complementary loss-of-function and gain-of-function genomic approaches provide a rapid means of investigating the function of candidate genes prior to initiating more cumbersome studies in vivo.
摘要
在本章中，我们描述了小鼠巨噬细胞功能基因组学研究的方法。特别地，我们描述了使用RNA干扰（RNAi）进行基因抑制和基因过表达的互补方法。这些方法易于应用于中高通量功能基因组学研究。这些互补的功能丧失和功能获得基因组方法为在开展更繁琐的体内研究之前研究候选基因的功能提供了一种快速手段。
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