Gandjeva Aneta, Tuder Rubin M
Program in Translational Lung Research, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA.
Methods Mol Biol. 2018;1809:315-329. doi: 10.1007/978-1-4939-8570-8_20.
The lung is ideally suited to the application of histological methods to study its structure, cellular composition, and molecular characteristics of more than 30 types of cells. The key in these endeavors are proper tissue preservation/fixation, well-established protocols aimed at sectioning and staining, and understanding of lung morphology. Molecular studies can be performed in laser-captured cells and microscopic structures.
肺非常适合应用组织学方法来研究其结构、细胞组成以及30多种细胞的分子特征。这些研究中的关键在于适当的组织保存/固定、成熟的切片和染色方案以及对肺形态学的理解。分子研究可以在激光捕获的细胞和微观结构中进行。