从人类胚胎干细胞分化生成心肌细胞

Cardiomyocyte Differentiation from Human Embryonic Stem Cells.

作者信息

Mazzotta Silvia, Lynch Adam T, Hoppler Stefan

机构信息

Institute of Medical Sciences, Foresterhill Health Campus, University of Aberdeen, Aberdeen, Scotland, UK.

出版信息

Methods Mol Biol. 2018;1816:67-78. doi: 10.1007/978-1-4939-8597-5_5.

DOI:10.1007/978-1-4939-8597-5_5

PMID:29987811

Abstract

In vitro generated human cardiomyocytes hold the ultimate promise for heart patients for repair of injured or diseased myocardium, but they also provide experimental models for studying normal cardiomyocyte development, for disease modeling and for drug development. Here we provide reliable protocols for differentiation of human embryonic stem cells into functional cardiomyocytes, together with Notes about troubleshooting and optimizing such protocols for specific cell lines. This chapter also briefly discusses other published protocols and those further adapted for differentiation of induced pluripotent stem cells into cardiomyocytes.

摘要

体外生成的人类心肌细胞为心脏病患者修复受损或患病心肌带来了最终希望，但它们也为研究正常心肌细胞发育、疾病建模和药物开发提供了实验模型。在这里，我们提供了将人类胚胎干细胞分化为功能性心肌细胞的可靠方案，以及关于针对特定细胞系对这些方案进行故障排除和优化的注意事项。本章还简要讨论了其他已发表的方案以及那些进一步适用于将诱导多能干细胞分化为心肌细胞的方案。