Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Kraków, Poland.
Methods Mol Biol. 2024;2835:69-82. doi: 10.1007/978-1-0716-3995-5_7.
Cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CMs) offer an attractive platform to evaluate the mechanisms of cardiovascular-related incidents and to develop and test new drugs for heart diseases. This work focuses on the comparison of two hiPSC-CM differentiation protocols: the GiWi method based on temporal modulation of the Wnt/β-catenin pathway and the commercially available PSC Cardiomyocyte Differentiation Kit. We underlined the need to optimize several parameters such as cell density or small molecule concentration (CHIR-99021, IWR-1) to obtain functional hiPSC-CMs. Both protocols yield a similar differentiation efficiency; therefore, the choice of a particular procedure may depend on the preferences of the experimenter.
人诱导多能干细胞（hiPSC-CMs）衍生的心肌细胞为评估心血管相关事件的机制以及开发和测试心脏病新药物提供了一个有吸引力的平台。本工作重点比较了两种 hiPSC-CM 分化方案：基于 Wnt/β-连环蛋白通路时间调节的 GiWi 方法和市售的 PSC 心肌细胞分化试剂盒。我们强调需要优化几个参数，如细胞密度或小分子浓度（CHIR-99021、IWR-1），以获得功能齐全的 hiPSC-CMs。两种方案的分化效率相似；因此，特定程序的选择可能取决于实验者的偏好。
