猴肾细胞感染SV40后富含大T抗原的病毒DNA复制位点

Large T antigen-rich viral DNA replication loci in SV40-infected monkey kidney cells.

作者信息

Harper F, Florentin Y, Puvion E

出版信息

Exp Cell Res. 1985 Dec;161(2):434-44. doi: 10.1016/0014-4827(85)90099-0.

Abstract

The nuclear distribution of the large T antigen (T-Ag) during lytic infection of CV1 monkey kidney cells with SV40 virus was studied by immunoelectron microscopy. The viral protein was associated with the cellular chromatin and also accumulated within a small number of clearly delimited areas of the nucleoplasm. These T-Ag-rich areas were devoid of viral particles but contain 3-10 nm DNA filaments in an amorphous matrix. We have named these areas 'viral DNA/T-Ag loci.' The combination of the immunostaining for T-Ag with ultrastructural autoradiography revealed that these viral DNA/T-Ag loci were the sites of active SV40 DNA synthesis. We suggest that the viral DNA/T-Ag loci may represent definite structural domains specifically involved in viral DNA replication regulated by SV40-T antigen.

摘要

利用免疫电子显微镜技术研究了猴肾CV1细胞被SV40病毒裂解感染期间大T抗原（T-Ag）的核分布情况。该病毒蛋白与细胞染色质相关，并且还积聚在核质中少数明确界定的区域内。这些富含T-Ag的区域没有病毒颗粒，但在无定形基质中含有3-10纳米的DNA细丝。我们将这些区域命名为“病毒DNA/T-Ag位点”。T-Ag免疫染色与超微结构放射自显影相结合显示，这些病毒DNA/T-Ag位点是活跃的SV40 DNA合成位点。我们认为，病毒DNA/T-Ag位点可能代表了由SV40-T抗原调控的、专门参与病毒DNA复制的特定结构域。