使用含脱氧肌苷的探针分离编码仙台病毒（HVJ）融合（F）糖蛋白的cDNA并进行测序。
Use of the deoxyinosine-containing probe to isolate and sequence cDNA encoding the fusion (F) glycoprotein of Sendai virus (HVJ).
作者信息
Miura N, Ohtsuka E, Yamaberi N, Ikehara M, Uchida T, Okada Y
出版信息
Gene. 1985;38(1-3):271-4. doi: 10.1016/0378-1119(85)90228-8.
A synthetic 20-mer based on the known amino acid (aa) sequence of the N-terminus of Sendai virus F1 polypeptide was synthesized. Using this dI-probe, which contained deoxyinosines at all six ambiguous codon positions, we isolated clones carrying cDNAs for the F mRNA of Sendai virus. Nucleotide (nt) sequence analysis revealed a long open reading frame (ORF) that encodes a protein of 565 aa. Thus, this type of dI-probes should prove useful for selecting cDNA clones, when the aa sequence is known and is characterized by high codon redundancy.
基于仙台病毒F1多肽N端已知氨基酸序列合成了一个20聚体的合成肽。使用这个在所有六个模糊密码子位置都含有脱氧次黄苷的dI探针，我们分离出了携带仙台病毒F mRNA cDNA的克隆。核苷酸（nt）序列分析揭示了一个长开放阅读框（ORF），它编码一个565个氨基酸的蛋白质。因此，当氨基酸序列已知且具有高密码子冗余特征时，这种类型的dI探针应该被证明对选择cDNA克隆是有用的。
Zotero 插件