枯草芽孢杆菌染色体复制终点terC的克隆与定位
Cloning and localization of the Bacillus subtilis chromosome replication terminus, terC.
作者信息
Smith M T, Aynsley C, Wake R G
出版信息
Gene. 1985;38(1-3):9-17. doi: 10.1016/0378-1119(85)90198-2.
A 10.9-kb segment of the Bacillus subtilis 168 chromosome has been cloned in an Escherichia coli plasmid and shown to contain terC (the replication terminus of the chromosome). The terC-containing portion of this plasmid has been subcloned within each of two overlapping fragments of DNA, 1.75 and 1.95 kb, again in E. coli plasmids. These have afforded a more precise definition of the location of terC in the B. subtilis chromosome and provided material for a detailed analysis of the structure and functioning of this site.
枯草芽孢杆菌168染色体的一个10.9千碱基片段已被克隆到大肠杆菌质粒中，并显示其包含terC（染色体的复制终点）。该质粒中含terC的部分已被亚克隆到两个重叠的DNA片段中，分别为1.75千碱基和1.95千碱基，同样是在大肠杆菌质粒中。这些操作更精确地确定了terC在枯草芽孢杆菌染色体中的位置，并为详细分析该位点的结构和功能提供了材料。
Zotero 插件