NHMRC Clinical Trials Centre, The University of Sydney , New South Wales , Australia.
Department of Gastrointestinal surgery, Strathfield Private Hospital , New South Wales , Australia.
Am J Physiol Endocrinol Metab. 2018 Oct 1;315(4):E634-E637. doi: 10.1152/ajpendo.00152.2018. Epub 2018 Jul 10.
Inappropriate insulin secretion from β-cells is considered as an early sign of impaired glucose tolerance and type 2 diabetes (T2D). Glucokinase (GCK) is an important enzyme that regulates glucose metabolism and ensures that the normal circulating glucose concentrations are maintained. GCK expression is induced by glucose and regulated via transcription factors and regulatory proteins. Recently, microRNA-206 (miR-206) was reported to regulate GCK and alter glucose tolerance in normal and high-fat diet-fed mice. Although the study findings have implications for human diabetes, studies in human islets are lacking. Here, we analyze human islets from individuals without or with T2D, using TaqMan-based real-time qPCR at the tissue (isolated islet) level as well as at single cell resolution, to assess the relationship between miR-206 and GCK expression in normal and T2D human islets. Our data suggest that, unlike mouse islets, human islets do not exhibit any correlation between miR-206 and GCK transcripts. These data implicate the need for further studies aimed toward exploring its potential role(s) in human islets.
β细胞胰岛素分泌异常被认为是糖耐量受损和 2 型糖尿病（T2D）的早期标志。葡萄糖激酶（GCK）是一种重要的酶，可调节葡萄糖代谢，确保维持正常的循环葡萄糖浓度。GCK 的表达受葡萄糖诱导，并通过转录因子和调节蛋白进行调节。最近，研究报道 microRNA-206（miR-206）可调节 GCK，并改变正常和高脂肪饮食喂养的小鼠的葡萄糖耐量。尽管这些研究结果对人类糖尿病有影响，但缺乏对人类胰岛的研究。在这里，我们使用基于 TaqMan 的实时 qPCR 技术，在组织（分离胰岛）水平以及单细胞分辨率水平，分析来自无 T2D 个体和 T2D 个体的人类胰岛，以评估 miR-206 和 GCK 在正常和 T2D 人类胰岛中的表达之间的关系。我们的数据表明，与小鼠胰岛不同，人类胰岛中 miR-206 和 GCK 转录本之间没有任何相关性。这些数据表明需要进一步研究，以探索其在人类胰岛中的潜在作用。
