Cao Yongsheng, Wang Di, Li Shaowu, Xu Liming, Zhao Jingzhuang, Liu Hongbai, Lu Tongyan, Zhang Qiya
Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Songfa Street No. 43, Daoli District, Harbin, 150070, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Songfa Street No. 43, Daoli District, Harbin, 150070, China.
Dev Comp Immunol. 2018 Nov;88:28-36. doi: 10.1016/j.dci.2018.07.004. Epub 2018 Jul 7.
MicroRNAs (miRNAs) are a class of regulators essential for numerous biological processes. Infectious hematopoietic necrosis virus (IHNV) is one of the most important viral pathogens in salmon and trout. In this study, the miRNA expression profiles of rainbow trout upon IHNV infection were explored. In total, 392 known miRNAs and 936 novel miRNAs were identified. Twelve known and 13 novel miRNAs were differentially expressed between infected and uninfected fish. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that certain miRNA target genes were associated with biological regulation, the immune system, and signal transduction. In addition, over- and suppressed expression of miR-146a-3p, miR-155-5p, miR-216a-5p, and miR-499b-5p could respectively increase and decrease viral gene expression in cells and viral titers. MiR-146a-3p and miR-216a-5p inhibited the expression of type-I IFN and the Mx1 gene induced by IHNV. These results provide preliminary insights into the IHNV-host interactions mediated by miRNAs.
微小RNA（miRNA）是一类对众多生物学过程至关重要的调节因子。传染性造血坏死病毒（IHNV）是鲑鱼和鳟鱼中最重要的病毒病原体之一。在本研究中，探索了虹鳟鱼感染IHNV后的miRNA表达谱。总共鉴定出392个已知miRNA和936个新miRNA。在感染和未感染的鱼之间，有12个已知miRNA和13个新miRNA差异表达。基因本体论和京都基因与基因组百科全书分析表明，某些miRNA靶基因与生物调节、免疫系统和信号转导有关。此外，miR-146a-3p、miR-155-5p、miR-216a-5p和miR-499b-5p的过表达和抑制表达可分别增加和降低细胞中的病毒基因表达及病毒滴度。miR-146a-3p和miR-216a-5p抑制了IHNV诱导的I型干扰素和Mx1基因的表达。这些结果为miRNA介导的IHNV与宿主相互作用提供了初步见解。
