School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, PR China; Key Laboratory for Water Quality Security and Protection in Pearl River Delta, Ministry of Education, Guangzhou 510006, PR China; Guangdong Provincial Key Laboratory of Radionuclides Pollution Control and Resources, Guangzhou 510006, PR China; Guangzhou University-Linköping University Research Center on Urban Sustainable Development, Guangzhou University, 510006 Guangzhou, PR China.
School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, PR China.
Bioresour Technol. 2018 Oct;266:447-453. doi: 10.1016/j.biortech.2018.07.012. Epub 2018 Jul 5.
Graphene oxide (GO) was introduced to Enterococcus avium strain BY7 sulfate-reducing bacteria culture as a carrier, GO was partially reduced by SRB to reduced graphene oxide (rGO). The rGO could further self-assemble Enterococcus avium strain BY7 sulfate-reducing bacteria to form BY-rGO particles. Growth and sulfate reduction activity of strain BY7 was promoted by rGO, which probably due to the protective effect of rGO, and enhanced electron transfer by rGO as electron shuttle. Effects of pH and temperature variance on strain BY-rGO were remarkably weakened, growth and sulfate reduction were observed from pH 2.0 to 12.0, and from 10 to 45 °C, respectively. Metal toxicity to BY7 strain SRB was sharply decreased in BY-rGO particles and heavy metal removal was remarkably accelerated (up to 50%). The immobilization methods established in this study might open a new way for the application of SRBs, especially under extreme environmental conditions.
氧化石墨烯（GO）被引入屎肠球菌 BY7 硫酸盐还原菌的培养物中作为载体，部分硫酸盐还原菌将 GO 还原为还原氧化石墨烯（rGO）。rGO 可以进一步将屎肠球菌 BY7 自组装成屎肠球菌 BY-rGO 颗粒。rGO 促进了 BY7 菌株的生长和硫酸盐还原活性，这可能是由于 rGO 的保护作用，以及 rGO 作为电子穿梭体增强了电子传递。pH 值和温度变化对 BY-rGO 的影响明显减弱，在 pH 值为 2.0 到 12.0 和 10 到 45°C 的范围内，均观察到了生长和硫酸盐还原。在 BY-rGO 颗粒中，重金属对 BY7 硫酸盐还原菌的毒性急剧降低，重金属去除速度显著加快（高达 50%）。本研究建立的固定化方法可能为硫酸盐还原菌的应用开辟新途径，特别是在极端环境条件下。
