Pharmaceutical Chemistry Department, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, ET 11562, Cairo, Egypt.
Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, ET 11562, Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Nov 5;204:743-747. doi: 10.1016/j.saa.2018.07.004. Epub 2018 Jul 4.
Alogliptin (ALO) and metformin (MET) are coformulated for the treatment of type II diabetes mellitus. ALO is estimated at its λ 277 nm (D), while MET was determined accurately by four spectrophotometric methods with minimum manipulation steps based on normalized division spectrum namely; ratio difference, advanced amplitude modulation, first derivative ratio (DD) and mean centering of the ratio spectra spectrophotometric methods. Linearity was acceptable over the concentration ranges of 5-40 and 2-16 μg/mL for ALO and MET, respectively. Accuracy and precision of the suggested methods were found to be within the acceptable limit. The specificity was inspected by analyzing laboratory prepared mixtures of the above drugs and their pharmaceutical preparation. The results of proposed and reported methods were statistically compared showing no significant difference regarding accuracy and precision. The developed methods could be applied for routine analysis of the cited drugs in quality control laboratories.
阿格列汀（ALO）和二甲双胍（MET）联合用于治疗 2 型糖尿病。ALO 在 277nm（D）处进行估算，而 MET 则通过四种分光光度法准确测定，这些方法的操作步骤最少，基于归一化除法光谱，即：比值差、高级调幅、一阶导数比（DD）和比值光谱的均值中心化分光光度法。对于 ALO 和 MET，线性范围分别在 5-40 和 2-16μg/mL 之间是可接受的。所建议方法的准确度和精密度均在可接受范围内。通过分析上述药物的实验室制备混合物及其药物制剂，检查了专属性。所提出的方法与已报道的方法的结果进行了统计学比较，在准确度和精密度方面没有显著差异。所开发的方法可应用于质量控制实验室中对上述药物的常规分析。
