Department of Medicine, Division of Nephrology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Central Arkansas Veterans Healthcare System, Little Rock, Arkansas, USA.
Nephron. 2018;140(2):129-133. doi: 10.1159/000491440. Epub 2018 Jul 10.
The search for acute kidney injury (AKI) biomarkers has identified a number of urine proteins that can be used to predict the presence of AKI but has struggled to identify proteins that are prognostic for severe AKI. In this review, we discuss 2 currently available biomarkers and the designs of the studies in which they were identified and relate this to the AKI characteristics they predict clinically. We discuss recent advances in mass spectrometry and sample preparation, which have improved the ability to identify low abundance proteins as well as the ability to characterize more of the protein by mass spectrometry. We show how these changes can lead to a deeper and more thorough analysis of the urine proteome. Finally, we highlight 2 important issues that can help in the identification of these biomarkers, appropriate study design and adequate technical characteristics in the analysis.
寻找急性肾损伤 (AKI) 的生物标志物已经确定了许多尿液蛋白质，这些蛋白质可用于预测 AKI 的存在，但难以识别对严重 AKI 具有预后价值的蛋白质。在这篇综述中，我们讨论了 2 种目前可用的生物标志物以及鉴定它们的研究设计，并将其与它们预测的 AKI 临床特征联系起来。我们讨论了质谱和样品制备方面的最新进展，这些进展提高了鉴定低丰度蛋白质的能力，以及通过质谱对更多蛋白质进行特征分析的能力。我们展示了这些变化如何导致对尿液蛋白质组的更深入和更彻底的分析。最后，我们强调了有助于鉴定这些生物标志物的 2 个重要问题，即适当的研究设计和分析中的足够技术特征。
