CSN6和Rab34参与小鼠睾丸支持细胞中的雄激素受体转运。
CSN6 and Rab34 Are Involved in Androgen Receptor Trafficking in Mouse Testicular Sertoli Cells.
作者信息
Deng Qiong, He Chihua, Wu Yong, Zhang Jianwen, Zhang Ying, Wang Zhu, Liang Hui, Yang Fei
机构信息
Department of Urology, The People's Hospital of Longhua, Shenzhen, China.
Reproductive Center, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, China.
出版信息
Cell Physiol Biochem. 2018;47(6):2360-2368. doi: 10.1159/000491608. Epub 2018 Jul 10.
BACKGROUND/AIMS: Androgen and its receptor (AR) play an important role in maintaining spermatogenesis and male fertility. Our previous studies showed that testosterone at a physiological concentration induces cytoplasmic AR translocation to the Sertoli cell plasma membrane of within 5 minutes.
METHODS
In this study, mass spectrometry (MS) and bioinformatic analyses were applied to identify candidate proteins mediating AR trafficking. The candidate proteins were knocked down by shRNA transfection.
RESULTS
Nine candidate proteins were identified by MS. The data was verified by co-immunoprecipitation and Western blot. Of the candidates, CSN6 regulated AR transport through the phosphorylation signaling pathway and Rab34 affected AR trafficking by regulating Ras activity.
CONCLUSIONS
CSN6 and Rab34 are involved in AR trafficking by regulating the phosphorylation signaling pathway. These findings provide new insights into the testosterone signaling pathway in Sertoli cells that mediates spermatogenesis.
背景/目的：雄激素及其受体（AR）在维持精子发生和男性生育能力方面发挥着重要作用。我们之前的研究表明，生理浓度的睾酮在5分钟内可诱导细胞质中的AR转运至支持细胞的质膜。
方法
在本研究中，应用质谱（MS）和生物信息学分析来鉴定介导AR转运的候选蛋白。通过shRNA转染敲低候选蛋白。
结果
通过MS鉴定出9种候选蛋白。通过免疫共沉淀和蛋白质印迹法验证了数据。在这些候选蛋白中，CSN6通过磷酸化信号通路调节AR转运，而Rab34通过调节Ras活性影响AR转运。
结论
CSN6和Rab34通过调节磷酸化信号通路参与AR转运。这些发现为支持细胞中介导精子发生的睾酮信号通路提供了新的见解。
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