Rhyner K, Taetle R, Bering H, To D
J Cell Physiol. 1985 Dec;125(3):608-12. doi: 10.1002/jcp.1041250333.
Cultured myeloid leukemia cells display transferrin receptors but decrease receptor display after differentiation induction or accumulation of intracellular iron. To determine whether regulation of transferrin receptors and ferritin were linked under these disparate conditions, serum-free and fetal bovine serum (FBS) cultures of HL60 promyelocytic leukemia cells were used to investigate relationships between transferrin receptor display and intracellular ferritin. Using 125I-transferrin binding and immunofluorescence staining for transferrin receptors, HL60 cells cultured in serum-free, transferrin-free medium expressed fewer transferrin receptors and contained increased ferritin when compared to cells cultured with FBS or transferrin supplemented, serum-free medium. When placed in medium containing transferrin, cells previously grown in transferrin-free medium rapidly re-expressed transferrin receptors and decreased their ferritin content. HL60 cells induced to differentiate into granulocytes or macrophages also decreased transferrin receptor display and increased their ferritin content. Transferrin receptor display and ferritin content in both proliferating and differentiating myeloid leukemia cells are inversely related and their regulation is closely linked. Regulation of transferrin receptor display and ferritin synthesis may be important events regulating myeloid cell growth and differentiation.
培养的髓系白血病细胞表达转铁蛋白受体，但在诱导分化或细胞内铁积累后受体表达减少。为了确定在这些不同条件下转铁蛋白受体和铁蛋白的调节是否相关联，利用HL60早幼粒细胞白血病细胞的无血清培养和胎牛血清（FBS）培养来研究转铁蛋白受体表达与细胞内铁蛋白之间的关系。通过¹²⁵I-转铁蛋白结合以及转铁蛋白受体的免疫荧光染色发现，与在补充了FBS或转铁蛋白的无血清培养基中培养的细胞相比，在无血清、无转铁蛋白培养基中培养的HL60细胞表达的转铁蛋白受体较少，且铁蛋白含量增加。当置于含有转铁蛋白的培养基中时，先前在无转铁蛋白培养基中生长的细胞会迅速重新表达转铁蛋白受体并降低其铁蛋白含量。诱导分化为粒细胞或巨噬细胞的HL60细胞也会减少转铁蛋白受体表达并增加其铁蛋白含量。在增殖和分化的髓系白血病细胞中，转铁蛋白受体表达和铁蛋白含量呈负相关，且它们的调节密切相关。转铁蛋白受体表达和铁蛋白合成的调节可能是调节髓系细胞生长和分化的重要事件。
