Kimura Yuki, Sasaki Motoki, Watanabe Kenichi, Dhakal Pramod, Sato Fumio, Taya Kazuyoshi, Nambo Yasuo
Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
J Equine Sci. 2018;29(2):33-37. doi: 10.1294/jes.29.33. Epub 2018 Jul 6.
Activin is secreted from equine uterine glands and plays important roles in establishment and maintenance of pregnancy in mares. This study aimed to localize activin receptors (ActRs) IA/B and IIA/B using immunohistochemistry in the uteroplacental tissues of seven pregnant Thoroughbred mares. At the time of tissue collection, the mares were at the following days of pregnancy: 88, 120, 161, 269, 290, 313, and 335 days. We fixed the uteroplacental tissues in 4% paraformaldehyde and obtained serial sections that were subsequently stained for analysis. All four isoforms of ActR were expressed in the uteroplacental tissues, including the endometrial epithelium, uterine glands, trophoblasts, and myometrium, throughout pregnancy. Our results suggested the potential role of activin in the uteroplacental tissues.
激活素由马属动物子宫腺体分泌，在母马妊娠的建立和维持中发挥重要作用。本研究旨在利用免疫组织化学方法，对7匹怀孕纯种母马的子宫胎盘组织中的激活素受体（ActRs）IA/B和IIA/B进行定位。在采集组织时，母马处于妊娠的以下天数：88天、120天、161天、269天、290天、313天和335天。我们将子宫胎盘组织固定在4%多聚甲醛中，获得连续切片，随后进行染色分析。在整个妊娠期，ActR的所有四种亚型均在子宫胎盘组织中表达，包括子宫内膜上皮、子宫腺体、滋养层细胞和子宫肌层。我们的结果提示了激活素在子宫胎盘组织中的潜在作用。
