Osmolovskiy Alexander A, Lukianova Anna A, Zvonareva Elena S, Kreyer Valeriana G, Baranova Nina A, Egorov Nikolay S
Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russian Federation.
Faculty of Biotechnology, Lomonosov Moscow State University, Moscow, Russian Federation.
Biotechnol Rep (Amst). 2018 Jun 18;19:e00265. doi: 10.1016/j.btre.2018.e00265. eCollection 2018 Sep.
A scheme for screening of micromycetes - producers of proteases with the activity of hemostasis system proteins, based on their enzymatic indices determination and the activity towards chromogenic peptide substrates for proteins of the hemostasis system was developed. Depending on the ability of proteases producers to cleave such substrates, an enzymatic reaction in conditions containing human plasma is suggested, which makes it possible to identify the potentiality of the target plasma hemostasis proenzymes activation.
基于对蛋白酶产生菌的酶学指标测定以及其对凝血系统蛋白质生色肽底物的活性，开发了一种筛选具有凝血系统蛋白质活性的蛋白酶产生菌的微真菌的方案。根据蛋白酶产生菌切割此类底物的能力，建议在含有人类血浆的条件下进行酶促反应，这使得识别目标血浆凝血前体酶激活的潜力成为可能。
