Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan.
Clin Exp Allergy. 2018 Dec;48(12):1726-1734. doi: 10.1111/cea.13231. Epub 2018 Aug 13.
Soya bean is a major food allergen in children. Component-resolved diagnostics has improved the accuracy of diagnosing immunoglobulin E (IgE)-mediated food allergies.
We aimed to develop a novel component for the diagnosis of soya bean allergy using recombinant technology.
Japanese paediatric patients with suspected soya bean allergy (n = 91) were included, and symptomatic (n = 40) and asymptomatic (n = 51) cases were divided through oral food challenge testing. Specific IgE (sIgE) antibodies to each recombinant allergen component were analysed by enzyme-linked immunosorbent assay, and the diagnostic performances of the components were assessed by area under the receiver operating characteristic curves (AUC).
Among the recombinant components, sIgE antibody levels to Gly m 8 showed the highest AUC (0.706). A combination of Gly m 8 and α' subunit of Gly m 5, improved the diagnostic performance of the single components. Moreover, the N-terminal extension region of α' subunit of Gly m 5, which has low cross-reactivity among the vicilins, showed higher diagnostic performance (AUC 0.695) than the full-length α' subunit of Gly m 5 (AUC 0.613). Based on these findings, we developed a fusion protein of Gly m 8 plus the extension region of α' subunit of Gly m 5; this fusion protein was very efficient for diagnosing soya bean allergy (AUC 0.801).
A fusion protein of Gly m 8 and the extension region of α' subunit of Gly m 5 could potentially diagnose soya bean allergy in paediatric patients. Fusion proteins may be useful for producing novel allergen components with improved diagnostic value.
大豆是儿童的主要食物过敏原。成分分辨诊断提高了 IgE 介导的食物过敏诊断的准确性。
我们旨在使用重组技术开发一种用于诊断大豆过敏的新型成分。
纳入了 91 例疑似大豆过敏的日本儿科患者，并通过口服食物挑战试验将有症状（n=40）和无症状（n=51）病例进行了区分。通过酶联免疫吸附试验分析了每种重组过敏原成分的特异性 IgE（sIgE）抗体，并用受试者工作特征曲线下面积（AUC）评估了成分的诊断性能。
在重组成分中，Gly m 8 的 sIgE 抗体水平显示出最高的 AUC（0.706）。Gly m 8 和 Gly m 5 的α'亚基的组合提高了单个成分的诊断性能。此外，在vicilins 中具有低交叉反应性的 Gly m 5 的α'亚基的 N 端延伸区显示出比全长的 Gly m 5 的α'亚基（AUC 0.613）更高的诊断性能（AUC 0.695）。基于这些发现，我们开发了 Gly m 8 加 Gly m 5 的α'亚基的延伸区的融合蛋白；该融合蛋白非常有效地用于诊断大豆过敏（AUC 0.801）。
Gly m 8 和 Gly m 5 的α'亚基的延伸区的融合蛋白可能有潜力诊断儿科患者的大豆过敏。融合蛋白可能有助于生产具有改进诊断价值的新型过敏原成分。
