a School of Public Health, Shandong University , Jinan , China.
b Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention; Shandong Provincial Center for Disease Control and Prevention , Jinan , China.
Hum Vaccin Immunother. 2018;14(10):2485-2490. doi: 10.1080/21645515.2018.1477911. Epub 2018 Jul 23.
Immune memory after hepatitis B vaccination among adults is still under investigation. In this study, adults who had normal and high antibody response to the primary series of hepatitis B vaccination (HepB) were followed up at 5 years after the primary immunization. A booster dose was given to those who had low hepatitis B surface antibody (anti-HBs) titers, defined as anti-HBs levels < 10 mIU/mL. Blood samples were collected at two weeks after the booster and anti-HBs levels were measured. We assumed those with ant-HBs levels > = 10 mIU/mL after the booster had anamnestic response. In total, 242 persons completed the booster and the anti-HBs test. The anamnestic response rate was 99.59% (241/242) and geometric mean concentration (GMC) of anti-HBs after the booster was 2989 mIU/mL (95% CI: 255, 35085). Anti-HBs titer after the booster dose had a positive correlation with anti-HBs titers measured right after the primary immunization as well as anti-HBs titers 5 years later just before the booster. After the booster, no significant difference was found in anti-HBs titers between participants who were immunized with the 10μg HepB vaccine and those with the 20μg vaccine. Multivariable analysis showed that 1) vaccine brand used for the primary vaccination, 2) anti-HBs titers after primary vaccination and 3) anti-HBs titers before the booster dose were independently associated with the anti-HBs titers after the booster 1) β = -0.21, 95% CI: -0.33, -0.09, P = 0.001; 2) β = 0.07, 95% CI: 0.05, 0.09, P < 0.001; 3) β = 0.04, 95% CI: 0.02, 0.07, P < 0.001). In summary, anamnestic response exists among almost all adults at five years after HepB primary immunization. Vaccine brand used for primary vaccination, initial anti-HBs titers after primary immunization and anti-HBs titers before the booster were the independent predictive factors of HepB anamnestic response titers.
乙型肝炎疫苗接种后免疫记忆仍在研究中。本研究对乙型肝炎疫苗（HepB）初免后抗体反应正常和高的成年人进行随访，在初免后 5 年进行加强免疫。对乙型肝炎表面抗体（抗-HBs）滴度较低者（< 10 mIU/mL）给予加强免疫。加强免疫后 2 周采集血样，检测抗-HBs 水平。我们假设加强免疫后抗-HBs 水平≥10 mIU/mL 者具有记忆反应。共有 242 人完成了加强免疫和抗-HBs 检测。记忆反应率为 99.59%（241/242），加强免疫后抗-HBs 几何平均浓度（GMC）为 2989 mIU/mL（95%CI：255，35085）。加强免疫后抗-HBs 滴度与初免后即刻及 5 年后加强免疫前的抗-HBs 滴度均呈正相关。加强免疫后，使用 10μg HepB 疫苗和 20μg 疫苗接种者的抗-HBs 滴度无显著差异。多变量分析显示，1）初免疫苗品牌、2）初免后抗-HBs 滴度、3）加强免疫前抗-HBs 滴度与加强免疫后抗-HBs 滴度独立相关 1）β=-0.21，95%CI：-0.33，-0.09，P=0.001；2）β=0.07，95%CI：0.05，0.09，P<0.001；3）β=0.04，95%CI：0.02，0.07，P<0.001）。综上所述，HepB 初免后 5 年，几乎所有成年人都存在记忆反应。初免疫苗品牌、初免后初始抗-HBs 滴度和加强免疫前抗-HBs 滴度是 HepB 记忆反应滴度的独立预测因素。
