Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Clínica Infantil, Ribeirão Preto, São Paulo, Brasil.
Universidade Federal de Sergipe, Departamento de Odontologia de Lagarto, Lagarto, Sergipe, Brasil.
J Appl Oral Sci. 2018 Jul 10;26:e20170512. doi: 10.1590/1678-7757-2017-0512.
To evaluate and correlate, in the same research, the mRNA expression and the staining of RANK, RANKL, OPG, TLR2 and MyD88 by immunohistochemistry in the apical periodontitis (AP) progression in mice.
AP was induced in the lower first molars of thirty-five C57BL/6 mice. They were assigned to four groups according to their euthanasia periods (G0, G7, G21 and G42). The jaws were removed and subjected to histotechnical processing, immunohistochemistry and real-time reverse transcription-PCR (qRT-PCR). Data were analyzed with parametric and nonparametric tests (α=0.05).
An increase of positive immunoreactivity for RANK, RANKL, OPG, TLR2 and MyD88 was observed over time (p<0.05). The RANKL expression was different between the groups G0 and G42, G21 and G42 (p=0.006), with G42 presenting the higher expression in both comparations. The OPG expression was statistically different between the groups G0 and G7, G7 and G21 and G7 and G42 (p<0.001), with G7 presenting higher expression in all the time points. The TLR2 expression was different between the groups G0 and G42 (p=0.03), with G42 showing the higher expression. The MyD88 expression presented a statistical significant difference between groups G7, G21 and G42 compared with G0 (p=0.01), with G0 presenting the smallest expression in all the comparisons. The Tnfrsf11/Tnfrsf11b (RANKL/OPG) ratio increased with the AP progression (p=0.002). A moderate positive correlation between MyD88 and RANKL (r=0.42; p=0.03) and between MyD88 and TLR2 (r=0.48; p<0.0001) was observed.
The expression of the RANK, RANKL, OPG, MyD88 and TLR2 proteins as well as the ratio Tnfrsf11/Tnfrsf11b (RANKL/OPG) increased with AP progression. There was also a moderate positive correlation between the expression Myd88-Tnfrsf11 and Tlr2-Myd88, suggesting the relevance of Tlr2-Myd88 in bone loss due to bacterial infection.
在同一研究中评估和关联 RANK、RANKL、OPG、TLR2 和 MyD88 的 mRNA 表达和免疫组织化学染色在小鼠根尖周炎（AP）进展中的作用。
在 35 只 C57BL/6 小鼠的下颌第一磨牙中诱导 AP。根据安乐死时间将它们分为四组（G0、G7、G21 和 G42）。取下颌骨并进行组织学处理、免疫组织化学和实时逆转录-PCR（qRT-PCR）。使用参数和非参数检验（α=0.05）分析数据。
随着时间的推移，观察到 RANK、RANKL、OPG、TLR2 和 MyD88 的阳性免疫反应性增加（p<0.05）。G0 和 G42、G21 和 G42 组之间的 RANKL 表达存在差异（p=0.006），G42 在这两种比较中均表现出更高的表达。OPG 表达在 G0 和 G7、G7 和 G21 以及 G7 和 G42 组之间存在统计学差异（p<0.001），G7 在所有时间点的表达均较高。TLR2 表达在 G0 和 G42 组之间存在差异（p=0.03），G42 表达较高。与 G0 相比，G7、G21 和 G42 组之间的 MyD88 表达存在统计学差异（p=0.01），G0 在所有比较中的表达最小。随着 AP 进展，Tnfrsf11/Tnfrsf11b（RANKL/OPG）比值增加（p=0.002）。观察到 MyD88 和 RANKL（r=0.42；p=0.03）以及 MyD88 和 TLR2（r=0.48；p<0.0001）之间存在中度正相关。
随着 AP 的进展，RANK、RANKL、OPG、MyD88 和 TLR2 蛋白的表达以及 Tnfrsf11/Tnfrsf11b（RANKL/OPG）比值增加。MyD88-Tnfrsf11 和 Tlr2-Myd88 之间也存在中度正相关，提示 Tlr2-Myd88 在细菌感染引起的骨丢失中具有相关性。
