Tikariha Hitesh, Khardenavis Anshuman A, Purohit Hemant J
Environmental Biotechnology and Genomics Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur, 440020, Maharashtra, India.
Arch Microbiol. 2018 Nov;200(9):1371-1379. doi: 10.1007/s00203-018-1551-x. Epub 2018 Jul 11.
Microbial community with their plasticity follows a course of changes that allow adaptation and survival in a particular habitat. In this study perturbations in microbial flora dwelling in two reactors with phenol as a carbon source under the limiting nitrogen and phosphorus conditions were monitored for 3 months with alterations of dissolved oxygen (DO). With the time, the shift in diversity and abundance of bacteria were observed with simultaneous increase in biofilm-forming bacteria like Pseudomonas, Escherichia, etc. Functional level screening revealed that the abundance of core metabolic genes were not much altered, however, the regulated level of increase in quorum sensing genes (acyl-homoserine lactone), biofilm-forming genes, catalase and ferroxidase enzymes at high DO suggest the survival mechanism of the community. This study sheds light on survival route followed by the bacterial community with abiotic stress, such as an increase in DO.
具有可塑性的微生物群落遵循着一系列变化过程，这些变化使得它们能够在特定栖息地中适应并生存。在本研究中，在限制氮和磷的条件下，以苯酚作为碳源，监测了两个反应器中微生物菌群的扰动情况，同时改变溶解氧（DO），持续监测了3个月。随着时间的推移，观察到细菌多样性和丰度发生了变化，同时形成生物膜的细菌如假单胞菌、大肠杆菌等数量增加。功能水平筛选显示，核心代谢基因的丰度变化不大，然而，在高溶解氧条件下，群体感应基因（酰基高丝氨酸内酯）、生物膜形成基因、过氧化氢酶和铁氧化酶的上调水平表明了该群落的生存机制。本研究揭示了细菌群落在诸如溶解氧增加等非生物胁迫下所遵循的生存途径。
