Biswas Sabarni, Biswas Asok K, De Bratati
a Centre for Advanced Study, Department of Botany , University of Calcutta , Kolkata India.
Plant Signal Behav. 2018;13(7):e1489670. doi: 10.1080/15592324.2018.1489670. Epub 2018 Jul 11.
Soil salinity has become a major concern for agriculture. Such constraints not only reinforce the urgent need to understand the underlying mechanisms by which plants cope during salt stress but also to develop cost-effective and farmer friendly halopriming technique to alleviate the adverse effects of salinity to some extent. Metabolomics approach was used to explore different responses to physiological metabolites and pathway variations that occur during salt stress responses in Cajanus cajan L. var. Rabi and to understand the role of halopriming in ameliorating stress at the level of metabolite. Seedlings raised from non-primed and haloprimed seeds, grown in hydroponic solution, were subjected to different concentrations of NaCl. After 21 days, metabolites were extracted, derivatized and analyzed by GC-MS. The data were analysed by different multivariate analyses. Chemometric study of the identified metabolites indicated that the leaves responded most to NaCl induced stress than the stem and root with production of beta-cyano-L-alanine and also increased level of different compatible solutes. O-Acetylsalicylic was also found to increase in all the parts upon facing stress but, such upregulated metabolite production was downregulated in the leaves when the seeds were haloprimed before germination, although many of the metabolites, including beta-cyanoalanine, showed a trend of increase with increase in salt concentrations. Important metabolites produced by C. cajan seedlings in response to salinity were unravelled. Pre-germination haloprimimg of seeds resulted in amelioration of NaCl induced stress, as the levels of stress induced metabolites were lowered.
土壤盐渍化已成为农业领域的一个主要问题。这些限制因素不仅凸显了迫切需要了解植物在盐胁迫下应对的潜在机制，还需要开发具有成本效益且对农民友好的盐引发技术，以在一定程度上减轻盐渍化的不利影响。采用代谢组学方法来探究木豆（Cajanus cajan L. var. Rabi）在盐胁迫反应期间对生理代谢物和途径变化的不同反应，并了解盐引发在代谢物水平上缓解胁迫的作用。将由未引发和盐引发种子培育出的幼苗种植在水培溶液中，使其遭受不同浓度的氯化钠处理。21天后，提取代谢物，进行衍生化处理并通过气相色谱 - 质谱联用仪进行分析。数据通过不同的多元分析方法进行分析。对已鉴定代谢物的化学计量学研究表明，叶片对氯化钠诱导的胁迫反应比茎和根更为明显，产生了β-氰基-L-丙氨酸，并且不同相容性溶质的水平也有所增加。还发现面对胁迫时，所有部位的邻乙酰水杨酸含量都会增加，但是当种子在萌发前进行盐引发处理时，叶片中这种上调的代谢物产生会下调，尽管许多代谢物，包括β-氰基丙氨酸，都呈现出随着盐浓度增加而增加的趋势。揭示了木豆幼苗对盐胁迫产生的重要代谢物。种子萌发前的盐引发处理减轻了氯化钠诱导的胁迫，因为胁迫诱导的代谢物水平降低了。
