Li L R, Zhang T, Wang S S, Niu Q
Institute of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2018 Mar 20;36(3):184-188. doi: 10.3760/cma.j.issn.1001-9391.2018.03.006.
To analyze the relationship between cognitive function and tumor necrosis factor receptor 1 (TNFR1) expression of occupational exposed workers to aluminum and provide the basis for the diagnosis of cognitive impairment. 140 cases Shanxi aluminum plant workers were collected in 2016 as the research object, including 70 potroom workers for exposure group, 70 non-electrolytic aluminum plant workers in the control group, respectively. Using mini mental status examination (MMSE), digit span test (DST), fuld object memory examination(FOME) and simple reaction time test(SRTT) evaluate the cognitive function of objects. Using graphite furnace atomic absorption method for the determination of plasma aluminum levels as an indicator of aluminum contact exposure of workers. Using RT-PCR method for detection of tumor necrosis factor receptor 1 (TNFR1) mRNA expression levels. And comparison group differences in cognitive and TNFR1 mRNA expression levels. The plasma aluminum content of exposed group (77.12±27.18) μg/L higher than the control group (55.6±28.69)μg/L (=0.000); Compared to control group, FOME and MMSE score was significantly increased in the exposed group (=0.000, =0.000), SRTT scores significantly higher in the exposed group (=0.001), DST no significant difference in the control group (=0.893). Compared to control group, The mRNA expression of TNFR1 was significantly higher in the exposed group(=0.002); Compared to control group, The protein expression of TNFR1 was significantly higher in the exposed group (=0.002). By correlation analysis in exposure group, plasma aluminum content was negatively correlated with MMSE and the DST (=-0.284, =-0.331, <0.05) and positively correlated with the SRTT, TNFR1 (mRNA) and TNFR1(protein)(=0.255, =0.333, =0.987, <0.01), MMSE was negatively related to TNFR1 (mRNA) and TNFR1 (protein) (=-0.268, =-0.255, <0.05); DST was negatively correlated with the SRTT and TNFR1 (protein)(=-0.267, =-0.330, <0.05); SRTT was positively correlated with TNFR1 (protein)(=0.243, <0.05); TNFR1 (mRNA) was positively correlated with TNFR1 (protein)(= 0.340, <0.01). Cognitive function change of occupational exposed workers to aluminum was related to the increase of TNFR1 expression.
分析职业性铝暴露工人认知功能与肿瘤坏死因子受体1（TNFR1）表达之间的关系，为认知功能障碍的诊断提供依据。选取2016年山西铝厂140名工人作为研究对象，其中电解车间工人70名为暴露组，非电解铝厂工人70名为对照组。采用简易精神状态检查表（MMSE）、数字广度测验（DST）、富尔德物体记忆测验（FOME）和简单反应时测试（SRTT）评估研究对象的认知功能。采用石墨炉原子吸收法测定血浆铝水平，作为工人铝接触暴露的指标。采用逆转录-聚合酶链反应（RT-PCR）法检测肿瘤坏死因子受体1（TNFR1）mRNA表达水平。比较两组认知功能及TNFR1 mRNA表达水平的差异。暴露组血浆铝含量（77.12±27.18）μg/L高于对照组（55.6±28.69）μg/L（P = 0.000）；与对照组相比，暴露组FOME和MMSE评分显著升高（P = 0.000，P = 0.000），SRTT评分显著升高（P = 0.001），DST评分差异无统计学意义（P = 0.893）。与对照组相比，暴露组TNFR1的mRNA表达显著升高（P = 0.002）；与对照组相比，暴露组TNFR1的蛋白表达显著升高（P = 0.002）。暴露组相关性分析显示，血浆铝含量与MMSE、DST呈负相关（r = -0.284，r = -0.331，P < 0.05），与SRTT、TNFR1（mRNA）和TNFR1（蛋白）呈正相关（r = 0.255，r = 0.333，r = 0.987，P < 0.01），MMSE与TNFR1（mRNA）和TNFR1（蛋白）呈负相关（r = -0.268，r = -0.255，P < 0.05）；DST与SRTT和TNFR1（蛋白）呈负相关（r = -0.267，r = -0.330，P < 0.05）；SRTT与TNFR1（蛋白）呈正相关（r = 0.243，P < 0.05）；TNFR1（mRNA）与TNFR1（蛋白）呈正相关（r = 0.340，P < 0.01）。职业性铝暴露工人认知功能改变与TNFR1表达增加有关。
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