Department of Occupational Health, School of Public Health, Shanxi Medical University, China.
Department of Occupational Health, School of Public Health, Shanxi Medical University, China; Key Lab of Environmental Hazard and Health of Shanxi Province, Shanxi Medical University, China; Key Lab of Cellular Physiology of Education Ministry, Shanxi Medical University, China.
Chemosphere. 2021 Sep;278:130484. doi: 10.1016/j.chemosphere.2021.130484. Epub 2021 Apr 3.
Aluminium (Al), not essential for biological activities, accumulates in the tissues. It exerts toxic effects on the nervous system, inducing in humans' irreversible cognitive impairment. In this study, a cluster sampling method was used to observe the cognitive function of long-term occupational Al-exposed workers in a large Al factory, and determine the expression of peripheral blood tumour necrosis factor receptor 1 (TNFR1), receptor-interacting protein 1 (RIP1), and RIP3. TNF-alpha, expressed in blood macrophages and microglia, with its receptors TNFR1, TR1 and TR3, enhances the necroptosis of neurons. Additionally, the relationship between the expression of TNFR1, RIP1, and RIP3 in the peripheral blood of long-term occupational Al-exposed workers and changes in their cognitive function was explored. The differences in the distributions of clock drawing test (CDT) scores among the three groups were statistically significant (P < 0.05). The results of correlation analysis showed that RIP1 and RIP3 protein contents were negatively correlated with mini-mental state examination (MMSE) and CDT scores (P < 0.05). Plasma Al content was positively correlated with other biological indicators (P < 0.05), and negatively correlated with MMSE and CDT scores (P < 0.05). Results showed that RIP3 protein had an incomplete mediation effect between plasma Al content and cognitive function. This suggests that Al may affect cognitive function by influencing the expression of TNFR1, RIP1, and RIP3 in the nervous system.
铝（Al）不是生物活动所必需的，会在组织中积累。它对神经系统有有毒作用，在人类中引起不可逆转的认知障碍。在这项研究中，采用聚类抽样方法观察了一家大型铝厂长期职业性铝暴露工人的认知功能，并确定了外周血肿瘤坏死因子受体 1（TNFR1）、受体相互作用蛋白 1（RIP1）和 RIP3 的表达。血液巨噬细胞和小胶质细胞中表达的 TNF-α与其受体 TNFR1、TR1 和 TR3 结合，增强神经元的坏死作用。此外，还探讨了长期职业性铝暴露工人外周血中 TNFR1、RIP1 和 RIP3 的表达与认知功能变化之间的关系。三组间画钟试验（CDT）评分的分布差异有统计学意义（P<0.05）。相关性分析结果表明，RIP1 和 RIP3 蛋白含量与简易精神状态检查（MMSE）和 CDT 评分呈负相关（P<0.05）。血浆铝含量与其他生物学指标呈正相关（P<0.05），与 MMSE 和 CDT 评分呈负相关（P<0.05）。结果表明，RIP3 蛋白在血浆铝含量与认知功能之间存在不完全中介效应。这表明铝可能通过影响神经系统中 TNFR1、RIP1 和 RIP3 的表达来影响认知功能。
